| Description |
1 online resource (xiv, 470 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 492 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; 492. 1064-3745
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Springer protocols (Series)
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| Contents |
Generation and analysis of multidimensional protein identification technology datasets / Selene K. Swanson, Laurence Florens, and Michael P. Washburn -- Quantitative peptide and protein profiling by mass spectrometry / Alexander Schmidt, Birgit Bisle, and Thomas Kislinger -- Label-free relative quantitation of prokaryotic proteomes using the accurate mass and time tag approach / Kim K. Hixson -- Classical proteomics: two-dimensional electrophoresis/MALDI mass spectrometry / Ursula Zimny-Arndt [and others] -- The use of difference in-gel electrophoresis for quantitation of protein expression / Rajat Sapra -- Liquid-chromatography-mass spectrometry of thylakoid membrane proteins / Christian G. Huber [and others] -- High accuracy mass spectrometry in large-scale analysis of protein phosphorylation / Jesper V. Olsen and Boris Macek -- Manual validation of peptide sequence and sites of tyrosine phosphorylation from MS/MS spectra / Amy M. Nichols and Forest M. White -- Assigning glycosylation sites and microheterogeneities in glycoproteins by liquid chromatography/tandem mass spectrometry / Yehia Mechref, Milan Madera, and Milos V. Novotny -- Structure analysis of N -glycoproteins / Stefanie Henning, Jasna Peter-Katalinicʹ, and Gottfried Pohlentz -- Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins / Christian Neusü€, and Matthias Pelzing -- Top-down proteomics on a high-field fourier transform ion cyclotron resonance mass spectrometer / Séverine A. Ouvry-Patat [and others] -- Capillary isoelectric focusing/reversed phase liquid chromatography/mass spectrometry / Cheng S. Lee and Brian M. Balgley -- Integrating accelerated tryptic digestion into proteomics workflows / Gordon W. Slysz and David C. Schriemer -- Hydrogen/deuterium exchange mass spectrometry / Xuguang Yan and Claudia S. Maier -- Mass spectrometry detection and characterization of noncovalent protein complexes / Sheng Yin and Joseph A. Loos -- Chemical cross-linking for protein-protein interaction studies / Xiaoting Tang and James E. Bruce -- Tissue analysis with high-resolution imaging mass spectrometry / A.F. Maarten Altelaar and Ron M.A. Heeren -- Proteomic global profiling for cancer biomarker discovery / Vitor Faca, Hong Wang, and Samir Hanash -- Analysis of protein glycosylation and phosphorylation using liquid phase separation, protein microarray technology, and mass spectrometry / Jia Zhao [and others] -- Transthyretin mass determination for detection of transthyretin familial amyloid / John F. O'Brien and H. Robert Bergen III -- Characterization of microorganisms by MALDI mass spectrometry / Catherine E. Petersen, Nancy B. Valentine, and Karen L. Wahl -- Mass spectrometric characterization of neuropeptides / Stephanie S. DeKeyser, James A. Dowell, and Lingjun Li -- Peptide and protein ion/ion reactions in electrodynamic ion traps: tools and methods / Scott A. McLuckey -- Electron capture dissociation LC/MS/MS for bottom-up proteomics / Roman A. Zubarev -- Two-dimensional ion mobility analyses of proteins and peptides / Alexandre A. Shvartsburg, Keqi Tang, and Richard D. Smith -- Proteomics for validation of automated gene model predictions / Kemin Zhou [and others] -- Support vector machines for improved peptide identification from tandem mass spectrometry database search / Bobbie-Jo M. Webb-Robertson |
| Summary |
When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fields from microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Staying faithful to the spirit of the Methods in Molecular Biology series, all chapters are written in the same format, whereby each chapter opens with a basic description of the method, followed by a description of the protocol and then practical step-by-step instructions to ensure experimental success. Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology |
| Notes |
Previous ed. published under title: Mass spectrometry of protein and peptides |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Proteins -- Analysis.
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Peptides -- Analysis
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Amino acid sequence.
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Mass spectrometry.
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Proteins -- analysis
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Mass Spectrometry -- methods
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Peptides -- analysis
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Amino Acid Sequence
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Mass Spectrometry
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mass spectrometry.
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Peptides -- Analysis.
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Proteins -- Analysis.
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Amino acid sequence.
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Mass spectrometry.
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Protéines -- Analyse.
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Spectroscopie de masse.
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Mass Spectrometry -- methods.
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Amino acid sequence
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Mass spectrometry
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Peptides -- Analysis
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Proteins -- Analysis
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Protéines -- Analyse.
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Spectroscopie de masse.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Lipton, Mary S.
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Paša-Tolić, Ljiljana.
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| ISBN |
9781934115480 |
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1934115487 |
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9781597454933 |
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1597454931 |
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