Title Sphingosine-1-Phosphate : methods and protocols / edited by Alice Pébay, Kursad Turksen

Published New York : Humana Press, ©2012

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Description 1 online resource (xi, 221 pages) Series Methods in molecular biology, 1064-3745 ; 874 Methods in molecular biology ; 874. Contents Biochemical methods for quantifying sphingolipids : ceramide, sphingosine, sphingosine kinase-1 activity, and sphingosine-1-phosphate / Leyre Brizuela and Olivier Cuvillier -- Isoform-selective assays for sphingosine kinase activity / Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson -- Quantification of sphingosine-1-phosphate and related sphingolipids by liquid chromatography coupled to tandem mass spectrometry / Constantin Bode and Markus H. Gräler -- Cleanup method for mass spectrometry of sphingosine-1-phosphate in blood and solid tissues using a phosphate capture molecule / Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi -- Immunohistochemical detection of sphingosine-1-phosphate and sphingosine kinase-1 in human tissue samples / Barbara Visentin, Gary Reynolds, and Roger Sabbadini -- Assessment of sphingosine-1-phosphate activity in biological samples by receptor internalization and adherens junction formation / Hideru Obinata and Timothy Hla -- High-throughput assays to measure intracellular Ca2+ mobilization in cells that express recombinant S1P receptor subtypes / William J. Valentine and Gabor Tigyi -- Quantifying sphingosine-1-phosphate-dependent activation of the RhoGTPases / Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack -- Optimized protocols to analyze sphingosine-1-phosphate signal transduction pathways during acrosomal exocytosis in human sperm / Silvia A. Belmonte and Laila Suhaiman -- Use of intravital microscopy and in vitro chemotaxis assays to study the roles of sphingosine-1-phosphate in bone homeostasis / Taeko Ishii [and others] -- Assessment of sphingosine-1-phosphate receptor expression and associated intracellular signaling cascades in primary cells of the human central nervous system / Veronique E. Miron [and others] -- Sphingosine-1-phosphate signaling in skeletal muscle cells / Elisabetta Meacci, Francesca Bini, and Chiara Battistini -- Maintenance of human embryonic stem cells by sphingosine-1-phosphate and platelet-derived growth factor / Raymond C.B. Wong, Martin F. Pera, and Alice Pébay -- Ceramide and sphingosine-1-phosphate signaling in embryonic stem cell differentiation / Erhard Bieberich -- Sphingosine-1-phosphate signaling in neural progenitors / Phillip Callihan and Shelley B. Hooks -- Utilizing sphingosine-1-phosphate to stimulate sprouting angiogenesis / Shih-Chi Su and Kayla J. Bayless Summary Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field Analysis Life sciences Cytology Cell culture Cell Biology celbiologie cellular biology celkweek sphingofosfolipiden sphingophospholipids fosfolipiden phospholipids Cellular Biology Celbiologie Notes Includes index Bibliography Includes bibliographical references and index Notes English Subject Sphingosine -- Laboratory manuals Glycols. Amino alcohols. Membrane lipids. Lipids. Alcohols. Amines. Organic compounds. Sphingosine. Sphingolipids. Glycols Amino Alcohols Membrane Lipids Lipids Alcohols Amines Chemicals and Drugs Organic Chemicals Sphingosine Sphingolipids lipid. organic compounds. Sphingolipids Organic compounds Membrane lipids Lipids Glycols Amino alcohols Amines Alcohols Sphingosine Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Pébay, Alice Turksen, Kursad ISBN 9781617798009 1617798002 1617797995 9781617797996

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