Cover; Half Title; Title Page; Copyright Page; Dedication; Table of Contents; 1 End Group Analysis of Proteins; Introduction; Reactions of the Amino Group; Reactions of the Carboxyl Group; End of Chapter Problems; 2 Limited Hydrolysis of Proteins; Introduction; Cyanogen Bromide; Trypsin; Chymotrypsin; Elastase; Pepsin; End of Chapter Problems; 3 Base Sequencing of DNA; Introduction; The Maxam-Gilbert Method; The Sanger Method; End of Chapter Problems; 4 Analysis of DNA by Restriction Mapping; Introduction; Single Enzyme Digests; Multiple Enzyme Digests; End of Chapter Problems
5 Additional Problems6 Adventures in Sequencing; Biocryptography â#x80;#x94; A New Science; A Night at the Opera (House); From Baltimore with Love; Summertime, and Is the Living Easy?; Appendix A Solutions to End of Chapter Problems; Appendix B Answers to Additional Problems; Index
Summary
The Logic of Biochemical Sequencing examines how to determine the primary structures of proteins and DNA and use them to stimulate the process of logical problem-solving. It concentrates on sequencing work and stresses the thought processes needed to make sense of what might otherwise be indecipherable data. The book also introduces "biocryptography," which serves as a basis for four short stories that use the results of sequence determinations to provide clues to higher order problems. Problems in the book range from elementary to difficult, and solutions to all problems are provided, many of them completely worked out. The book is an excellent supplementary text for students in a full-year biochemistry course, as well as for biochemists and molecular biologists
