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Title Androgen action : methods and protocols
Published Totowa, NJ : Humana Press, 2011


Description 1 online resource (XI, 386 pages 68 illustrations, 12 illustrations in color.)
Series Methods in molecular biology Androgen action
Methods in molecular biology (Clifton, N.J.) ; v. 776. 1064-3745
Contents Molecular Mechanisms of Androgen Action -- A Historical Perspective -- Androgen Action during Prostate Carcinogenesis -- Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry -- Ligand Competition Binding Assay for the Androgen Receptor -- Analysis of Androgen Receptor Activity by Reporter Gene Assays -- Identification and Characterization of Androgen Response Elements -- In vitro and In vivo Silencing of the Androgen Receptor -- Analysis of Interdomain Interactions of the Androgen Receptor -- Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells -- FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells -- Analysis of Nuclear Receptor Acetylation -- Analysis of Androgen Receptor SUMOylation -- Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor -- Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF -- Global Identification of Androgen Response Elements -- Tissue Specific Knockout of Androgen Receptor in Mice -- Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting -- Automated Microscopy and Image Analysis for Androgen Receptor Function -- Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer -- Regulation of Apoptosis by Androgens in Prostate Cancer Cells -- Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association -- Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells
Summary Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states
Bibliography Includes bibliographical references and index
Notes English
Subject Androgens -- Physiology
Androgens -- Pathophysiology.
Generative organs, Male -- Diseases.
Nuclear receptors (Biochemistry)
Genitourinary organs -- Diseases.
Transcription factors.
DNA-binding proteins.
Biological assay.
Prostate -- Diseases.
Receptors, Steroid
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Genital Diseases, Male
Urogenital Neoplasms
Receptors, Cytoplasmic and Nuclear
Hormones, Hormone Substitutes, and Hormone Antagonists
Male Urogenital Diseases
Neoplasms by Site
Physiological Effects of Drugs
Transcription Factors
DNA-Binding Proteins
Amino Acids, Peptides, and Proteins
Pharmacologic Actions
Chemical Actions and Uses
Chemicals and Drugs
Receptors, Androgen
Biological Assay
Prostatic Neoplasms
Prostatic Diseases
Investigative Techniques
Genital Neoplasms, Male
Female Urogenital Diseases
Transcription factors
Prostate -- Diseases
Nuclear receptors (Biochemistry)
Genitourinary organs -- Diseases
Generative organs, Male -- Diseases
DNA-binding proteins
Biological assay
Androgens -- Pathophysiology
Human Anatomy & Physiology.
Health & Biological Sciences.
Animal Biochemistry.
Form Electronic book
Author Saatcioglu, Fahri, contributor
ISBN 9781617792434
Other Titles Androgen Action: Methods and Protocols
Methods in Molecular Biology, Volume 776