| Description |
1 online resource (xi, 358 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; volume no. 1520 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1520. 1064-3745
|
| Contents |
Antibiotics : precious goods in changing times / Peter Sass -- Mining bacterial genomes for secondary metabolite gene clusters / Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert -- Production of antimicrobial compounds by fermentation / Henrik Harms, Gabriele M. König, and Till F. Schäberle -- Structure elucidation of antibiotics by NMR spectroscopy / Georgios Daletos, Elena Ancheeva, Raha S. Orfali, Victor Wray, and Peter Proksch -- Computer-aided drug design methods / Wenbo Yu and Alexander D. MacKerell Jr. -- Cytotoxicity assays as predictors of the safety and efficacy of antimicrobial agents / Alexander Zipperer and Dorothee Kretschmer -- Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds / Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard -- Determination of bacterial membrane impairment by antimicrobial agents / Miriam Wilmes and Hans-Georg Sahl -- Mass-sensitive biosensor systems to determine the membrane interaction of analytes / Sebastian G. Hoss and Gerd Bendas -- Measurement of cell membrane fluidity by Laurdan GP : fluorescence spectroscopy and microscopy / Kathi Scheinpflug, Oxana Krylova, and Henrik Strahl -- In vitro assays to identify antibiotics targeting DNA metabolism / Allan H. Pang, Sylvie Garneau-Tsodikova, and Oleg V. Tsodikov -- Fluorescence-based real-time activity assays to identify RNase P inhibitors / Yu Chen, Xin Liu, Nancy Wu, and Carol A. Fierke -- Reporter gene-based screening for TPP riboswitch activators / Christina E. Lünse and Günter Mayer -- Cell-based fluorescent screen to identify inhibitors of bacterial translation initiation / Federica Briani -- Bacterial histidine kinases : overexpression, purification, and inhibitor screen / Mike Gajdiss, Michael Türck, and Gabriele Bierbaum -- Expression profiling of antibiotic-resistant bacteria obtained by laboratory evolution / Shingo Suzuki, Takaaki Horinouchi, and Chikara Furusawa -- Sample preparation for mass-spectrometry based absolute protein quantification in antibiotic stress research / Florian Bonn, Sandra Maass, and Dörte Becher -- Label-free quantitation of ribosomal proteins from Bacillus subtilis for antibiotic research / Sina Schäkermann, Pascal Prochnow, and Julia E. Bandow -- Functional metagenomics to study antibiotic resistance / Manish Boolchandani, Sanket Patel, and Gautam Dantas -- Epidemiological surveillance and typing methods to track antibiotic resistant strains using high throughput sequencing / Miguel Paulo Machado, Bruno Ribeiro-Gonçalves, Mickael Silva, Mário Ramirez, and João André Carriço |
| Summary |
This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocols, viewed November 28, 2016) |
| Subject |
Antibiotics -- Laboratory manuals
|
|
Anti-Bacterial Agents
|
|
Antibiotics
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Sass, Peter (Research Associate), editor.
|
| ISBN |
9781493966349 |
|
1493966340 |
|
1493966324 |
|
9781493966325 |
|
