Title Oxidative stress biomarkers and antioxidant protocols / edited by Donald Armstrong

Published Totowa, N.J. : Humana Press, ©2002

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Description 1 online resource (xiv, 322 pages) : illustrations Series Methods in molecular biology ; v. 186 Methods in molecular biology (Clifton, N.J.) ; v. 186 Contents Human xanthine oxidoreductase determination by a competitive elisa / M.G. Battelli and S. Musiani -- Simultaneous determination of polyunsaturated fatty acids and corresponding monohydroperoxy and monohydroxy peroxidation products by HPLC / R.W. Browne and D. Armstrong -- Determination of products of lipid oxidation by infrared spectroscopy / D. Borchman and S. Sinha -- Detection of docosahexaenoic acid hydroperoxides in retina by gas chromatography/mass spectrometry / G.S. Wu and N.A. Rao -- Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies / Y. Kato and T. Osawa -- Techniques for determining the metabolic pathways of eicosanoids and for evaluating the rate-controlling enzymes / N. Panesar, Y.G. Deshpande and D.L. Kaminski -- Mass spectrometric quantification of f2-isoprostanes as indicators of oxidant stress / J.D. Morrow and L.J. Roberts, 2nd -- Formation of apolipoprotein ai-aii heterodimers by oxidation of high-density lipoprotein / A.S. Moses and G.A. Francis -- Detection of certain peroxynitrite-induced DNA modifications / H. Ohshima [and others] -- Hydroxyl and 1-hydroxyethyl radical detection by spin trapping and gc-ms / J.A. Castro and G.D. Castro -- Analysis of aliphatic amino acid alcohols in oxidized proteins / B. Morin [and others] -- Rapid determination of glutamate using HPLC technology / A. Afzal [and others] -- A rapid method for the quantification of gsh and gssg in biological samples / M. Afzal [and others] -- Protein carbonyl measurement by elisa / I.H. Buss and C.C. Winterbourn -- N epsilon-(carboxymethyl)lysine (cml) as a biomarker of oxidative stress in long-lived tissue proteins / J.N. Shaw, J.W. Baynes and S.R. Thorpe -- Measurement of S-glutathionated hemoglobin in human erythrocytes by isoelectric focusing electrophoresis / H.W. Chen and C.K. Lii -- Oxidation of cellular DNA measured with the comet assay / A.R. Collins and M. Dusinska -- Measurement of DNA double-strand breaks with giant DNA and high molecular-weight DNA fragments by pulsed-field gel electrophoresis / Y. Higuchi -- Evaluation of antibodies against oxygen free radical-modified DNA by elisa / R. Ali and K. Alam -- Simultaneous analysis of multiple redox-active metabolites from biological matrices / B.S. Kristal, K. Vigneau-Callahan and W.R. Matson -- Determination of uric acid in urine by fast-scan voltammetry (FSV) using a highly activated carbon fiber electrode / R. Bravo, D.M. Stickle and A. Brajter-Toth -- Measurement of [alpha]-tocopherol turnover in plasma and in lipoproteins using stable isotopes and gas chromatography/mass spectrometry / E.J. Parks -- Analysis of tocotrienols in different sample matrixes by HPLC / K. Sundram and R.M. Nor -- Measurement of [beta]-carotene 15,15'-dioxygenase activity by reverse-phase HPLC / A. During, A. Nagao and J.C. Smith, Jr. -- Ubiquinol/ubiquinone ratio as a marker of oxidative stress / Y. Yamamoto and S. Yamashita -- Catechol- and pyrogallol-type flavonoids. Analysis of tea catechins in plasma / K. Umegaki, M. Sano and I. Tomita -- Pyruvate dehydrogenase complex as a marker of mitochondrial metabolism. Inhibition by 4-hydroxy-2-nonenal / M.S. Patel and L.G. Korotchkina -- Ceruloplasmin detection by SDS-PAGE, immunoblotting, and in situ oxidase activity / L.A. Levin -- Metallothionein determination by isocratic HPLC with fluorescence derivatization / S. Miyairi and A. Naganuma -- Quantification of oxidized metallothionein by a CD-saturation method / D. Klein [and others] -- Fractionation of herbal medicine for identifying antioxidant activity / M. Afzal and D. Armstrong -- Designing safer (soft) drugs by avoiding the formation of toxic and oxidative metabolites / N. Bodor and P. Buchwald -- Statistical correction of the area under the ROC curve in the presence of random measurement error and applications to biomarkers of oxidative stress / E.F. Schisterman Summary Oxidative stress has been linked to a variety of medical problems, including cardiovascular disease, cancer, arthritis, diabetes, and aging. Drawing on the growing volume of research into the impact of oxidative stress on pathophysiological mechanisms, Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the perturbations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format by accomplished investigators from prestigious universities, institutes, and laboratories around the world, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltammetry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols (Humana, 2002), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays. Cutting-edge and highly practical, Oxidative Stress Biomarkers and Antioxidant Protocols provides many powerful new tools for detecting oxidative stress and for investigating the possibilities for therapeutic intervention monitoring in a variety of serious diseases Bibliography Includes bibliographical references and index Notes English Print version record Subject Free radicals (Chemistry) -- Pathophysiology -- Laboratory manuals Antioxidants -- Physiological effect -- Laboratory manuals Oxidative stress -- Pathophysiology -- Research -- Laboratory manuals Oxidative Stress -- physiology Antioxidants -- metabolism Free Radicals -- metabolism MEDICAL -- Pathology. MEDICAL -- Pathophysiology. Antioxidants -- Physiological effect Free radicals (Chemistry) -- Pathophysiology Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Armstrong, Donald, 1931- ISBN 9781592591732 0896038505 9780896038509 0585403333 9780585403335 1592591736 1280821353 9781280821356 9786610821358 6610821356

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