| Description |
1 online resource (xvii, 741 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1880 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1880. 1064-3745
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| Contents |
Recombinant expression, purification, and assembly of p62 filaments / Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, and Carsten Sachse -- Structural studies of autophagy-related proteins / Melanie Schwarten, Oliver H. Weiergr€aber, Dusˇan Petrović,Birgit Strodel, and Dieter Willbold -- Structural studies of mammalian autophagy lipidation complex / Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, and Takanori Otomo -- Structural studies of selective autophagy in yeast / Akinori Yamasaki, Yasunori Watanabe, and Nobuo N. Noda -- Biophysical studies of LC3 family proteins / Javier H. Herva s, Zurine Anton, and Alicia Alonso -- Reconstituting autophagy initiation from purified components / Peter Mayrhofer and Thomas Wollert -- Cell-free reconstitution of autophagic membrane formation / Min Zhang and Liang Ge -- Use of peptide arrays for identification and characterization of LIR motifs / Mads Skytte Rasmussen, Asa Birna Birgisdottir, and Terje Johansen -- Studying autophagic lysosome reformation in cells and by an in vitro reconstitution System / Yang Chen, Qian Peter Su, and Li Yu -- Formation of autophagosomes coincides with relaxation of membrane curvature / Jaime Agudo-Canalejo and Roland L. Knorr -- Studies of receptor-Atg8 interactions during selective autophagy / Christine Abert and Sascha Martens -- Correlative light and electron microscopy of autophagosomes / Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, and Eeva-Liisa Eskelinen -- Improved electron microscopy fixation methods for tracking autophagy-associated membranes in cultured mammalian cells / Ritsuko Arai and Satoshi Waguri -- Three-color simultaneous live imaging of autophagy-related structures / Hiroyuki Ueda, Ouin Kunitaki, and Maho Hamasaki -- Correlative live-cell imaging and super-resolution microscopy of autophagy / Eleftherios Karanasios -- Methods for imaging autophagosome dynamics in primary neurons / Audrey Dong, Vineet Vinay Kulkarni, and Sandra Maday -- Imaging autophagy in hiPSC-derived midbrain dopaminergic neuronal cultures for parkinson's disease research / Petros Stathakos, Natalia Jimenez-Moreno, Lucy Crompton, Paul Nistor, Maeve A. Caldwell, and Jon D. Lane -- Correlative light and electron microscopy to analyze LC3 proteins in Caenorhabditis elegans embryo / Céline Largeau and Renaud Legouis -- Imaging noncanonical autophagy and LC3-associated phagocytosis in cultured cells / Elise Jacquin, Katherine Fletcher, and Oliver Florey -- Measurement of bulk autophagy by a cargo sequestration assay / Nikolai Engedal, Morten Luhr, Paula Szalai, and Per O. Seglen -- Methods to detect loss of lysosomal membrane integrity / Sonja Aits -- Monitoring of autophagy and cell volume regulation in kidney epithelial cells in response to fluid shear stress / Maria M. Lazari, Idil Orhon, Patrice Codogno, and Nicolas Dupont -- Identification and regulation of multimeric protein complexes in autophagy via SILAC-based mass spectrometry approaches / Stéphanie Kaeser-Pebernard, Britta Diedrich, and Jorn Dengjel -- Identification and validation of novel autophagy regulators using an endogenous readout siGENOME screen / Maria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-Ichi Sakamaki, Kevin M. Ryan, Michael Howell, and Sharon A. Tooze -- Autophagy pathway mapping to elucidate the function of novel autophagy regulators identified by high-throughput screening / Martina Wirth and Sharon A. Tooze -- In vitro screening platforms for identifying autophagy modulators in mammalian cells / Elena Seranova, Carl Ward, Miruna Chipara, Tatiana R. Rosenstock, and Sovan Sarkar -- Automated detection of autophagy response using single cell-based microscopy assays / Amelie J. Mueller and Tassula Proikas-Cezanne -- Methods for the study of entotic cell death / Jens C. Hamann, Sung Eun Kim, and Michael Overholtzer -- MHC class I internalization via autophagy proteins / Monica Loi, Laure-Anne Ligeon, and Christian Mu¨nz -- Analysis of autophagy for liver pathogenesis / Nazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, and Xiao-Ming Yin -- Autophagy in 3D in vitro and ex vivo cancer models / Carlo Follo, Dario Barbone, William G. Richards,Raphael Bueno, and V. Courtney Broaddus -- Autophagy in platelets / Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie, Jinchao Zhang, Sidney W. Whiteheart, and Qing Jun Wang -- Methods to image macroautophagy in the brain in vivo / Xigui Chen, Kanoh Kondo, and Hitoshi Okazawa -- Measuring nonselective and selective autophagy in the liver / Takashi Ueno and Masaaki Komatsu -- Measuring autophagy in pancreatitis / Alejandro Ropolo, Daniel Grasso, and Maria I. Vaccaro -- Characterization of the "Autophagic Flux" in prostate cancer tissue biopsies by LC3A/LAMP2a immunofluorescence and confocal microscopy / D. Kalamida, A. Giatromanolaki, and M. I. Koukourakis -- Methods to determine the role of autophagy proteins in C. elegans aging / Sivan Henis-Korenblit and Alicia Meléndez -- Investigating non-selective autophagy in Drosophila / Szabolcs Taká ts, Sarolta Toth, Gyo˝zo˝ Szenci, and Gá bor Juhá sz -- Imaging the dynamics of mitophagy in live cells / Andrew S. Moore and Erika L. F. Holzbaur -- Triggering mitophagy with photosensitizers / Cheng-Wei Hsieh and Wei Yuan Yang -- Investigating mitophagy and mitochondrial morphology in vivo using mito-QC : a comprehensive guide / Thomas G. McWilliams and Ian G. Ganley -- Assays to monitor mitophagy in Drosophila / Panagiotis Tsapras, Anne-Claire Jacomin, and Ioannis P. Nezis -- Mitophagy dynamics in Caenorhabditis elegans / Konstantinos Palikaras, Eirini Lionaki, and Nektarios Tavernarakis -- Methods for studying mitophagy in yeast / Panagiota Kolitsida and Hagai Abeliovich -- Measuring antibacterial autophagy / Keith B. Boyle and Felix Randow -- Quantitative phosphoproteomics of selective autophagy receptors / Thomas Juretschke, Petra Beli, and Ivan Dikic -- Analysis of chaperone-mediated autophagy / Y. R. Juste and A. M. Cuervo -- Interactive autophagy : monitoring a novel form of selective autophagy by macroscopic observations / Jana Petri and Roland L. Knorr |
| Summary |
This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed January 7, 2019) |
| Subject |
Cell death -- Laboratory manuals
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Apoptosis -- Laboratory manuals
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Autophagy
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Apoptosis
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Cell death
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
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| Author |
Ktistakis, Nicholas T., editor
|
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Florey, Oliver, editor
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| ISBN |
9781493988730 |
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1493988735 |
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