Title Apoptosis methods in pharmacology and toxicology : approaches to measurement and quantification / edited by Myrtle A. Davis

Published Totowa, N.J. : Humana Press, ©2002

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Description 1 online resource (xiv, 156 pages) : illustrations Series Methods in pharmacology and toxicology, 1940-6053 Methods in pharmacology and toxicology. 1557-2153 Contents Apoptosis Methods in Pharmacology and Toxicology -- Series Foreword -- Preface -- Contents -- Contributors -- Introduction -- Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death -- Analysis of Apoptosis by Laser-Scanning Cytometry -- Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections -- DNA Microarrays -- Microarray Analysis of Apoptosis -- ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases -- Index Summary Apoptosis, or programmed cell death, is a widespread cellular process that regulates numerous important biological events ranging from the metamorphosis of the tadpole tail to the elimination of surplus brain cells in the formation of proper synaptic connections. In Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification, Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful in toxicology and pharmacology research. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high-throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems. Presented by authors well-versed in the technical problems and challenges in analyzing apoptosis, each method explicates its successful use, describes its limitations, and shows how it may be applied in large-scale screening operations. Concise and eminently practical, Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification offers pharmacologists, toxicologists, pathologists, and many other biomedical scientists today's gold standard reference source for methods that definitively identify and accurately quantify apoptosis Bibliography Includes bibliographical references and index Notes English Print version record Subject Apoptosis -- Research -- Methodology Cytology -- Technique. Apoptosis -- physiology Cytological Techniques SCIENCE -- Life Sciences -- Anatomy & Physiology. Cytology -- Technique Apoptosis -- Research -- Methodology Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Davis, Myrtle A. ISBN 9781592592791 1592592791 1280830646 9781280830648 9786610830640 6610830649

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