| Description |
1 online resource (xi, 306 pages) : illustrations |
| Series |
Methods in molecular medicine ; 22 |
|
Methods in molecular medicine ; 22. 1543-1894
|
| Contents |
Introduction to polymerase chain reaction / Mark C. Miller and Laura Cunningham -- Methodological issues in polymerase chain reaction for RNA / Alessandra D. Rasmussen -- Subtractive hybridization of cDNA libraries / Gregory S. Kelner and Richard A. Maki -- The use of differential display RT-PCR for identifying altered gene expression in ischemic brain injury / Xinkang Wang and Giora Z. Feuerstein -- Analysis of gene expression by multiprobe RNase protection assay / Anna K. Stalder [and others] -- Molecular probes for PNS neurotoxicity, degeneration, and regeneration / Arrel D. Toews -- Mobility-shift DNA-binding assay using gel electrophoresis / Yang Xiao and Keith Pennypacker -- Transfection of mammalian cells in vitro: use in analysis of neuronal damage / Melvin L. Billingsley and Theresa A. Thompson -- Detection of apoptotic cells in the vervous system -- Terrie Kagan and Zahra Zakeri -- Translocation assays of protein kinase C activation / Ginger G. Wescott, Christina M. Manring, and David M. Terrian -- Astrocyte and neuron coculturing method / Michael Aschner and Barbara A. Bennett -- Viability assays for cells invitro: the ethidium/calcium assay and the immunofluorescence combination assay / Theresa A. Thompson -- Measurement of the nitric oxide synthase activity using the citrulline assay / Thomas R. Ward and William R. Mundy -- Measurement of intraneuronal free calcium using the fluorescencent probe technique / Prasada Rao S. Kodavanti -- Measurement of calcium buffering by intracellular organelles in brain / Prasada Rao S. Kodavanti -- Receptor-mediated release of inositolphosphates in brain slices / Pushpa Tandon -- The use of enzyme-linked immunosorbent assays to quantitate proteins in biological functions / Dori R. Germolec -- Assays for the analysis of synaptic proteins in neurodegenerative disorders / Eliezer Masliah and Michael Alford -- ZZymographic method for the measurement of monoamine neurotransmitters in microdialysis perfusates using HPLC-ECD / Ian Acworth and Michael L. Cunningham -- Biochemical measurement of cholinesterase activity / Stephanie Padilla, T. Leon Lassiter, and Deborah Hunter -- Functional study of glutamate receptor channels in brain slices / Susan Jones and Jerrel L. Yakel -- Selective silver staining for neuronal degeneration / Jeanene K. Olin and Karl F. Jensen -- Immunohistological markers of cerebrovascular integrity / Karl F. Jensen [and others] -- Electron probe X-ray microanalysis: a quantitative electron microscopy technique for measurement of elements and water in nervous tissue cells / Richard M. LoPachin and Christopher L .Gaughan |
| Summary |
In Neurodegeneration Methods and Protocols, Jean Harry and Hugh Tilson bring together a collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. The methods span a multidisciplinary range of cellular and molecular approaches to both normal and injured nervous system function, and particularly to neurodegenerative processes. Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques. Cutting-edge and authoritative, Neurodegeneration Methods and Protocols will illuminate nervous system function, injury, degeneration, and the repair/regenerative process. These powerful techniques will also allow established laboratories-both experimental and clinical-to expand their array of methods for solving research problems and help utilize multidisciplinary procedures beyond their experience |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| In |
Springer Protocols |
| Subject |
Nervous system -- Degeneration -- Research -- Methodology
|
|
Neurons -- Research -- Methodology
|
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Molecular neurobiology -- Methodology
|
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Apoptosis -- Research -- Methodology
|
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Nervous system -- Degeneration.
|
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Nerve Degeneration
|
|
Neurodegenerative Diseases
|
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Nervous system -- Degeneration
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Apoptosis -- Research -- Methodology
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Molecular neurobiology -- Methodology
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Harry, Jean.
|
|
Tilson, Hugh A.
|
| ISBN |
9781592596041 |
|
1592596045 |
|
