| Description |
1 online resource (xi, 253 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1064-3745 ; 1162 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1162. 1064-3745
|
| Contents |
Axon Regeneration: What Needs to Be Overcome? -- Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitro -- The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance Related Intracellular Signaling in Live Dorsal Root Ganglia Neuronal Growth Cones -- Neurite Outgrowth and Growth Cone Collapse Assays to Assess Neuronal Responses to Extracellular Cues -- Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants -- Growth Cone Collapse Assay -- Axon Length Quantification Microfluidic Culture Platform for Growth and Regeneration Study -- Organotypic Slice Co-Culture Systems to Study Axon Regeneration in the Dopaminergic System Ex Vivo -- Monitoring Neuron and Astrocyte Interactions with a 3D Cell Culture System -- Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury -- Polymeric Biomaterials for Nerve Regeneration: Fabrication and Implantation of a Biodegradable Nerve Guide -- A Highly Reproducible Mouse Model of Compression Spinal Cord Injury -- Using Templated Agarose Scaffolds to Promote Axon Regeneration Through Sites of Spinal Cord Injury -- In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration -- Assessing Motor Outcome and Functional Recovery Following Nerve Injury -- The Use of an Adeno-Associated Viral Vector for Efficient Bicistronic Expression of Two Genes in the Central Nervous System -- Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues -- Time-Lapse In Vivo Imaging of Dorsal Root Nerve Regeneration in Mice -- Using Manganese-Enhanced MRI to Assess Optic Nerve Regeneration |
| Summary |
Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies. After an introductory section, this detailed volume continues with sections focusing on axon growth in vitro, providing a range of protocols that can be used to examine intracellular signalling pathways, axonal responses to extracellular factors, and methods for quantifying outgrowth |
|
The next section provides protocols for inducing experimental injury in vivo as well as some highly promising protocols for promoting regeneration, which segues into the final section highlighting a series of protocols that can be used to monitor the extent of axon regeneration in vivo, ranging from tract tracing to in vivo imaging and functional recovery |
|
As a book in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
|
Practical and reliable, Axon Growth and Regeneration: Methods and Protocols aims to serve researchers studying axon regeneration with a significant set of diverse tools, vital for moving on to the next generation of exciting new discoveries in the field |
| Analysis |
biomedische wetenschappen |
|
biomedicine |
|
neurowetenschap |
|
neuroscience |
|
neurobiologie |
|
neurobiology |
|
Medicine (General) |
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Geneeskunde (algemeen) |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 6, 2014) |
| Subject |
Axons -- Regeneration -- Laboratory manuals
|
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Axons -- Growth -- Laboratory manuals
|
|
Axons -- physiology
|
|
Axons -- Growth
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Murray, Andrew J., editor
|
| ISBN |
9781493907779 |
|
1493907778 |
|
149390776X |
|
9781493907762 |
|
