| Description |
1 online resource (xi, 289 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 271 |
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Methods in molecular biology (Clifton, N.J.) ; v. 271
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| Contents |
Characterization of B lymphopoiesis in mouse bone marrow and spleen / Richard R. Hardy and Susan A. Shinton -- Characterization of B-cell maturation in the peripheral immune system / Rita Carsetti -- Identification of B-cell subsets : an exposition of 11-color (Hi-D) FACS methods / James W. Tung [and others] -- Analysis of B-cell life-span and homeostasis / Irmgard Fürster -- Differentiation of B lymphocytes from hemotopoietic stem cells / Paulo Vieira and Ana Cumano -- Analysis of lymphocyte development and function using the rag-deficient blastocyst complementation system / Faith M. Young, Carl A. Pinkert, and Andrea Bottaro -- Conditional gene mutagenesis in B-lineage cells / Stefano Casola -- Analysis of the germinal center reaction and in vivo long-lived plasma cells / Kai-Michael Toellner, Mahmood Khan, and Daniel Man-Yuen Sze -- T-cell-dependent immune responses, germinal center development, and the anaysis of V-gene sequences / Claudia Berek |
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Introduction of genes into primary murine splenic B cells using retrovirus vectors / Kuo-I Lin and Kathryn Calame -- Immunoglobulin class switching: in vitro induction and analysis / Sven Kracker and Andreas Radbruch -- In vitro and in vivo assays of B-lymphocyte migrations / Chantal Moratz and John H. Kehrl -- Analysis of antigen-specific B-cell memory directly ex vivo / Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams -- B-cell signal transduction : tyrosine phosphorylation, kinase activity, and calcium mobilization / Tilman Brummer [and others] -- Isolation of lipid rafts from B lymphocytes / Anu Cherukuri [and others] -- Molecular single-cell PCR analysis of rearranged immunoglobulin genes as a tool to determine the clonal composition of normal and malignant human B cells -- Analysis of B-cell immune tolerance induction using transgenic mice / Helen Ferry and Richard J. Cornall -- Analysis of B-cell signaling using dt40 B cell line / Tomoharu Yasuda and Tadashi Yamamoto |
| Summary |
Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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UofL: Multi-user access (3 concurrent users) |
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Print version record |
| In |
Springer Protocols |
| Subject |
B cells -- Laboratory manuals
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B cells.
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B-Lymphocytes
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MEDICAL -- Immunology.
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B cells
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Gu, Hua, PhD.
|
|
Rajewsky, K., 1936-
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| ISBN |
9781592597963 |
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1592597963 |
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1588293475 |
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9781588293473 |
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1280360070 |
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9781280360077 |
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9786610360079 |
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6610360073 |
|
