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E-book

Title Regulatory B cells : methods and protocols / edited by Gaetano Vitale and Francesca Mion
Published New York, NY : Humana Press, 2014

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Description 1 online resource (xi, 292 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; Volume 1190
Methods in molecular biology (Clifton, N.J.) ; v. 1190. 1064-3745
Summary Annotation Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology
Analysis celkweek
cell culture
biomedische wetenschappen
biomedicine
immunologie
immunology
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed July 23, 2014)
Subject B cells -- Laboratory manuals
B-Lymphocytes, Regulatory
B cells
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Vitale, Gaetano, editor.
Mion, Francesca, editor.
ISBN 9781493911615
1493911619
1493911600
9781493911608