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Title Bacterial protein secretion systems : methods and protocols / editors Laure Journet and Eric Cascales
Published New York, NY : Humana Press : Springer, ©2017


Description 1 online resource (xv, 528 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; v. 1615
Methods in molecular biology (Clifton, N.J.) ; v. 1615. 1064-3745
Contents Identification of protein secretion systems in bacterial genomes using MacSyFinder / Sophie S. Abby and Eduardo P.C. Rocha -- Protein sorting prediction / Henrik Nielsen -- Cell fractionation / Melissa Petiti, Laetitia Houot, and Denis Duché -- Defining lipoprotein localisation by fluorescence microscopy / Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée -- Identification of lipoproteins using globomycin and radioactive palmitate / Nienke Buddelmeijer -- Defining membrane protein localization by isopycnic density gradients / Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow -- Cell surface exposure / Anna Konovalova -- Probing inner membrane protein topology by proteolysis / Maxence S. Vincent and Eric Cascales -- Mapping of membrane protein topology by substituted cysteine accessibility method (SCAM) / Mikhail Bogdanov -- Defining membrane protein topology using pho-lac reporter fusions / Gouzel Karimova and Daniel Ladant -- In vivo and in vitro protein-peptidoglycan interactions / Gang Li and S. Peter Howard -- Measure of peptidoglycan hydrolase activity / Yoann G. Santin and Eric Cascales -- Protein-protein interaction : Bacterial Two-Hybrid / Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant -- Protein-protein interactions : yeast two-hybrid system / Jer-Sheng Lin and Erh-Min Lai -- Protein-protein interactions : cytology two-hybrid / Krishnamohan Atmakuri -- Fusion reporter approaches to monitoring transmembrane helix interactions in bacterial membranes / Laureen Logger, Abdelrahim Zoued, and Eric Cascales -- Protein-protein interactions : co-immunoprecipitation / Jer-Sheng Lin and Erh-Min Lai -- Protein-protein interaction : tandem affinity purification in bacteria / Julie P.M. Viala and Emmanuelle Bouveret -- Site-directed and time-resolved photocrosslinking in cells metabolically labeled with radioisotopes / Raffaele Ieva -- Protein-protein interactions : pull-down assays / Arthur Louche, Suzana P. Salcedo, and Sarah Bigot -- Protein-protein interactions : surface plasmon resonance / Badreddine Douzi -- Assessing energy-dependent protein conformational changes in the TonB system / Ray A. Larsen -- Defining assembly pathways by fluorescence microscopy / Abdelrahim Zoued and Andreas Diepold -- Large complexes : cloning strategy, production, and purification / Eric Durand and Roland Lloubes -- Shearing and enrichment of extracellular type IV pili / Alba Katiria Gonzalez Rivera and Katrina T. Forest -- Blue native PAGE analysis of bacterial secretion complexes / Susann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner -- In situ imaging of bacterial secretion systems by electron cryotomography / Gregor L. Weiss, João M. Medeiros, and Martin Pilhofer -- Structural analysis of protein complexes by cryo electron microscopy / Tiago R.D. Costa, Athanasios Ignatiou, and Elena V. Orlova -- Bacterial filamentous appendages investigated by solid-state NMR spectroscopy / Birgit Habenstein and Antoine Loquet -- Energy requirements for protein secretion via the flagellar type III secretion system / Marc Erhardt -- Identification of effectors : precipitation of supernatant material / Nicolas Flaugnatti and Laure Journet -- Screening for secretion of the type VI secretion system protein Hcp by enzyme-linked immunosorbent assay and colony blot / Brent S. Weber, Pek Man Ly, and Mario F. Feldman -- Effector translocation : cya reporter assay / Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko -- Monitoring effector translocation using the TEM-1 beta-lactamase reporter system / Julie Allombert, Anne Vianney, and Xavier Charpentier -- Effector translocation assay : differential solubilization / Irina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota -- Quantitative determination of anti-bacterial activity during bacterial co-culture / Juliana Alcoforado Diniz, Birte Hollmann, and Sarah J. Coulthurst
Summary This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed July 5, 2017)
Subject Bacterial proteins -- Laboratory manuals
Bacteriology -- Technique.
Medical laboratory technology.
Bacteriological Techniques
Bacterial Proteins -- metabolism
Clinical Laboratory Techniques
Microbiology (non-medical)
Science -- Life Sciences -- Biology -- Microbiology.
Medical laboratory technology
Bacteriology -- Technique
Bacterial proteins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Journet, Laure, editor
Cascales, Eric, editor
ISBN 9781493970339