| Description |
1 online resource (xxvi, 308 pages) : illustrations |
| Series |
Laboratory companion.
|
| Contents |
Ch. 1. Cholera Toxin: Mechanism of Action and Potential Use in Vaccine Development / G.-F. Zhang, W.A. Patton and J. Moss -- Ch. 2. Cholera Toxin and Escherichia coli Heat-labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities / W.A. Patton, G.-F. Zhang and J. Moss -- Ch. 3. Pertussis Toxin / C. Locht and R. Antoine -- Ch. 4. Pertussis Toxin as a Cell Biology Tool / B. Nurnberg -- Ch. 5. Clostridium botulinum ADP-ribosyltransferase C3 / K. Aktories and G. Koch -- Ch. 6. Clostridium botulinum C3 Exoenzyme and Studies on Rho Proteins / C.D. Nobes and A. Hall -- Ch. 7. Preparation of Clostridium botulinum C3 Exoenzyme and Application of ADP-ribosylation of Rho Proteins in Biological Systems / Y. Saito and S. Narumiya -- Ch. 8. Actin-ADP-ribosylating Toxins: Cytotoxic Mechanisms of Clostridium botulinum C2 Toxin and Clostridium perfringens Iota Toxin / K. Aktories, P. Sehr and I. Just -- Ch. 9. Purification, Activation and Endocytosis of Botulinum C2 Toxin / I. Ohishi -- Ch. 10. The Role of the Clostridium botulinum C2 Toxin as a Research Tool to Study Eucaryotic Cell Biology / I.L. Simpson -- Ch. 11. Probing the Actin Cytoskeleton by Clostridium botulinum C2 Toxin and Clostridium perfringes Iota Toxin / K. Aktories, U. Prepens and P. Sehr -- Ch. 12. Clostridium difficile Toxins / M. Thelestam, I. Florin and E. Chaves-Olarte -- Ch. 13. Clostridium difficile Toxin B as a Probe for Rho GTPases / I. Just, J. Selzer and F. Hofmann -- Ch. 14. Clostridial Neurotoxins / G. Schiavo and C. Montecucco -- Ch. 15. Isolated Nerve Terminals as a Model System for the Study of Botulinum and Tetanus Toxins / J. Blasi, E. Link and R. Jahn -- Ch. 16. Clostridial Toxins and Endocrine Secretion: Their Use in Insulin-Secreting Cells / J. Lang, R. Regazzi and C.B. Wollheim -- Ch. 17. Pore-forming Toxins / S. Bhakdi, A. Valeva and I. Walev -- Ch. 18. Application of Alpha-toxin and Streptolysin O as Tools in Cell Biological / G. Ahnert-Hilger and U. Weller -- Ch. 19. Toxins as Transporting Tools / S. Olsnes, O. Klingenberg and R. Munoz -- Ch. 20. A Brief Guide to the Safe Handling of Biological Toxin / A.B. Maksymowych and L.L. Simpson |
| Summary |
This is a survey of well characterized and recently discovered bacterial protein toxins. Leading investigators of the respective toxins review the various molecular mechanisms of action, ranging from toxin-induced ADP-ribosylation up to membrane perforation by pore-forming toxins. Thy also describe the consequences on host physiology before focusing on potential applications as cell biological and pharmacological tools for research and medical applications. Detailed descriptions of the methodology include the engineering and use of modified and chimeric toxins for better performance. |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Bacterial Toxins. -- Laboratory manuals
|
|
Bacterial Toxins.
|
| Genre/Form |
Laboratory Manual.
|
|
Handbook.
|
| Form |
Electronic book
|
| Genre/Form |
Laboratory manuals.
|
| Author |
Aktories, K.
|
| ISBN |
9783527614615 |
|
3527614613 |
|
9783527614608 |
|
3527614605 |
|
