| Description |
1 online resource (x, 247 pages) : illustrations |
| Series |
Methods in molecular biology ; 321 |
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Methods in molecular biology (Clifton, N.J.) ; v. 321
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| Contents |
Overview of advances in microfluidics and microfabrication / Shelley D. Minteer and Christine M. Moore -- Introduction to microfabrication techniques / Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou -- Fabrication of PDMS microfluidics using SU-8 molds / Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou -- Fabrication of microelectrodes using the lift-off technique / Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou -- Laser ablation as a fabrication technique for microfluidic devices / Emanual Waddell -- Reversible, room temperature bonding of glass devices for microfluidics / Loranelle L. Lockyear -- Solid phase extraction in packed beds on glass microdevices / Loranelle L. Lockyear -- Fabrication of micromachined magnetic particle separators for bioseparation in microfluidic systems / Jin Woo Choi -- Hydrogel-immobilized antibodies for microfluidic immunoassays / Gloria Thomas [and others] |
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PCR in miniaturized systems : big progress in little devices / Kalee D. Spitzack and Victor Ugaz -- Polymerase chain reaction (PCR) on microchips / Maria C. Carles -- A sensitive sandwich DNA array using fluorescent nanoparticle probes / Xiajun Zhao and Yanfu Yuan -- Microfluidic ethanol biobatteries on a microchip / Shelley D. Minteer and Christine M. Moore -- Microfluidic chambers for cell migration and neuroscience research / Anne M. Taylor, Seog Woo Rhee, and Noo Li Jeon -- Microtextured polydimethylsiloxane substrates for culturing mesenchymal stem cells / Erik T.K. Peterson and Ian Papautsky -- Contraction study of a single cardiac muscle cell in the microfluidic chip / Xiujun Li and Paul Li -- Microscale inegrated sperm sorter / Yaokuang Chung [and others] |
| Summary |
Over the last ten years, miniaturization has dramatically shrunk the size of both cellular telephones and chemical instrumentation, and now also holds the promise of developing portable, inexpensive, and disposable analytical systems on a microchip. In Microfluidic Techniques: Reviews and Protocols, hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, polymerase chain reaction (PCR), immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and informative, Microfluidic Techniques: Reviews and Protocols synthesizes the chemistry, biology, and engineering underlying microfluidic devices so that molecular biologists, as well as analytical and clinical chemists, can advance easily from macroscale to microscale biochemical and bioanalytical techniques |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Biochips -- Laboratory manuals
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Microfluidics -- Laboratory manuals
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Microfluidics -- instrumentation
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Miniaturization -- methods
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Microfluidics -- methods
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Microchip Analytical Procedures -- methods
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TECHNOLOGY & ENGINEERING -- Biomedical.
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MEDICAL -- Family & General Practice.
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MEDICAL -- Allied Health Services -- Medical Technology.
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MEDICAL -- Biotechnology.
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MEDICAL -- Lasers in Medicine.
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Biochips
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Microfluidics
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Minteer, Shelley D
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| ISBN |
1588295176 |
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9781588295170 |
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1592599974 |
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9781592599974 |
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1280358637 |
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9781280358630 |
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9786610358632 |
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661035863X |
|
