Title Oxidants and antioxidants : ultrastructure and molecular biology protocols / edited by Donald Armstrong

Published Totowa, N.J. : Humana Press, ©2002

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Description 1 online resource (xvi, 356 pages) : illustrations Series Methods in molecular biology ; v. 196 Methods in molecular biology (Clifton, N.J.) ; v. 196 Contents Cytochemical localization of H2O2 in biological tissues / E.A. Ellis and M.B. Grant -- Localization of intracellular lipid hydroperoxides using the tetramethylbenzidine reaction for transmission electron microscopy / E.A. Ellis [and others] -- The immunohistochemical localization of glutathione peroxidase / K. Akeo, T. Hiramitsu and K. Watanabe -- 4-hydroxy-2-nonenal (4-HNE) staining by anti-HNE antibody / H.J. Majima, T. Nakanishi-Ueda and T. Ozawa -- Immunohistochemical detection of protein oxidation / J. Frank, A. Pompella and H.K. Biesalski -- Indirect immunofluorescence detection of protein-bound 4-hydroxynonenal in tissue sections and isolated cells / A. Pompella [and others] -- Methods for studying the binding of advanced glycated proteins to receptors for advanced glycation endproducts (AGE receptors) / P.J. Thornalley -- Measurement of blue fluorescence as a protein marker for oxidized membranes / K. Kikugawa -- X-ray diffraction analysis of membrane structure changes with oxidative stress / R.P. Mason and R.F. Jacob -- Detection of estrogen receptor by in situ hybridization / K. Kobayashi and H. Kobayashi -- Tumor necrosis factor-[alpha] quantification and expression by in situ hybridization / T.A. Ignatowski and R.N. Spengler -- Translocation of p47phox and activation of nadph oxidase in mononuclear cells / A. Aljada, H. Ghanim and P. Dandona -- Activation of nuclear factor-[kappa] b (NF-[kappa] b) in mononuclear cells (MNC) / A. Aljada, H. Ghanim and P. Dandona -- Assay of intracellular hydrogen peroxide generation in activated individual neutrophils by flow cytometry / Y. Ito and D.A. Lipschitz -- DNA-binding activity of hypoxia-inducible factors (HIFS) / G. Camenisch, R.H. Wenger and M. Gassmann -- Analysis of heat-shock transcription factor and element-binding activity / Y.J. Lee -- Slot-blot hybridization in studying gene expression during oxygen deprivation / E. Ma and G.G. Haddad -- An expression system for a transporter of iron and other metals / M.D. Garrick and K.G. Dolan -- Analysis of gene expression following oxidative stress / D.R. Crawford [and others] -- Measurement of immunoglobulin G oxidation by western-blot analysis / A. Chow [and others] -- Thioredoxin and redox regulation of the nuclear receptor / Y. Makino, K. Okamoto and H. Tanaka -- Analysis of phospholipid hydroperoxide glutathione peroxidase mRNA / X.G. Lei and W.H. Cheng -- Expression of human phospholipid hydroperoxide glutathione peroxidase / K. Yagi, S. Komura and N. Ohishi -- Superoxide production in the islet of langerhans detected by the mcla chemiluminescence method / T. Sakurai and S. Terakawa -- In vivo detection of transition metals and nitrosyl-heme complexes using ex vivo electron paramagnetic resonance spectroscopy / D.M. Hall and G.R. Buettner -- Spectral-spatial electron paramagnetic resonance imaging (EPRI) in skin biopsies at 9.5 GHz / J. Fuchs, N. Groth and T. Herrling -- In vivo in situ detection of nitric oxide using low-frequency EPR spectroscopy / P. Tsai [and others] -- Introduction of NOS II gene into primary cultures of bovine and human endothelial cells / G.L. Cao [and others] -- Detection of peroxynitrite-induced protein and DNA modifications / S. Lorch [and others] -- Immunochemical detection of a fluorophore derived from the lipid peroxidation product 4-hydroxy-2-nonenal and lysine / P.A. Szweda, L. Tsai and L.I. Szweda -- Liver slice technology as an in vitro model for metabolic and toxicity studies / S. Thohan and G.M. Rosen -- Cytofluorescence techniques for the visualization of distinct pools of protein thiols at the single cell level / A. Pompella [and others] -- Preparation of microspheres and incorporation of lipid hydroperoxide for sustained release studies / D. Armstrong [and others] -- Measurement of carotenoids in the living primate eye using resonance raman spectroscopy / P.S. Bernstein and W. Gellermann -- Interstitial photodynamic therapy with moving exposure fiber / T. Hirano [and others] -- Statistical analysis. Receiver operating characteristic (roc) curve and lipid peroxidation / E.F. Schisterman Summary It is now feasible to identify subcellular sites where oxidative stress reactions occur and to determine gene expression relevant to those complex pathways. In Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, a panel of world authorities present cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, X-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47phox translocation, and microsphere delivery systems. The collection emphasizes the visual observation of free radicals or products altered by oxidative stress, and includes numerous in vivo, ex vivo, and in vitro applications. Two companion volumes, Oxidative Stress Biomarkers and Antioxidant Protocols (2002), and Free Radical and Antioxidant Protocols (1998), focus on the related biochemical and cellular biological methods. Authoritative and highly practical, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols provides a comprehensive collection of powerful ultrastructure and molecular tools for the evaluation of metabolic disorders linked to oxidative stress and the aging process Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Oxidation, Physiological -- Laboratory manuals Antioxidants -- Laboratory manuals Oxidative stress. Antioxidants. Histocytochemistry -- methods Oxidative Stress Antioxidants -- chemistry Biomarkers -- chemistry Oxidants -- chemistry Oxidants Antioxidants antioxidant. MEDICAL -- Pathology. MEDICAL -- Pathophysiology. Antioxidants Oxidation, Physiological Oxidatieve stress. Antioxidanten. Wetenschappelijke technieken. Genre/Form Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Armstrong, D. ISBN 9781592592746 1592592740 0896038513 9780896038516 128083059X 9781280830594 9786610830596 6610830592

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