Limit search to available items
Book Cover

Title Fluorescent protein-based biosensors : methods and protocols / edited by Jin Zhang and Qiang Ni, Robert H. Newman
Published New York. : Humana Press : Springer, ©2014


Description 1 online resource (xi, 251 pages) : illustrations (some color)
Series Methods in molecular biology ; 1071
Methods in molecular biology (Clifton, N.J.) ; v. 1071. 1064-3745
Contents Design and application of genetically encodable biosensors based on fluorescent proteins / Robert H. Newman and Jin Zhang -- Introduction to fluorescence imaging techniques geared towards biosensor applications / J. Goedhart, Mark A. Hink, and Kees Jalink -- Quantitative measurement of Ca² and Zn² in mammalian cells using genetically encoded fluorescent biosensors / J. Genevieve Park and Amy E. Palmer -- Detecting cAMP with an epac-based FRET sensor in single living cells / J. Klarenbeek and Kees Jalink -- Analysis of compartmentalized cAMP : a method to compare signals from differently targeted FRET reporters / Alessandra Stangherlin [and others] -- Genetically encoded fluorescent biosensors for live cell imaging of lipid dynamics / Moritoshi Sato -- Live-cell imaging of cytosolic NADH-NAD redox state using a genetically encoded fluorescent biosensor / Yin Pun Hung and Gary Yellen -- Measuring membrane voltage with microbial rhodopsins / Adam E. Cohen and Daniel R. Hochbaum -- Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons / Ana F. Oliveira and Ryohei Yasuda -- Imaging kinase activity at protein scaffolds / Maya T. Kunkel and Alexandra C. Newton -- Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells / Sohum Mehta and Jin Zhang -- Genetically encoded FRET indicators for live-cell imaging of histone acetylation / Kazuki Sasaki and Minoru Yoshida -- Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity / Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang -- Biosensor imaging in brain slice preparations / Marina Polito, Pierre Vincent, and Elvire Guiot -- Optical calcium imaging using DNA-encoded fluorescence sensors in transgenic fruit flies, Drosophila melanogaster / Shubham Dipt, Thomas Riemensperger, and André Fiala -- Multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel / Nwe-Nwe Aye-Han and Jin Zhang -- FRET and BRET-based biosensors in live cell compound screens / Katie Herbst Robinson, Jessica R. Yang, and Jin Zhang -- Integrating fluorescent biosensor data using computational models / Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman
Summary In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena
Analysis eiwitten
life sciences
cell physiology
Proteins and Enzymes
Eiwitten en enzymen
Bibliography Includes bibliographical references and index
Notes English
Subject Biosensors -- Laboratory manuals
Fluorescent probes -- Laboratory manuals
Cell physiology.
Life sciences.
Physical sciences.
Cellular signal transduction.
Molecular Probe Techniques
Investigative Techniques
Publication Formats
Biochemical Phenomena
Cell Physiological Phenomena
Biological Science Disciplines
Publication Characteristics
Chemical Phenomena
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Natural Science Disciplines
Phenomena and Processes
Amino Acids, Peptides, and Proteins
Disciplines and Occupations
Chemicals and Drugs
Laboratory Manuals
Green Fluorescent Proteins
Signal Transduction
Biosensing Techniques
Luminescent Proteins
biological sciences.
physical sciences.
Physical sciences
Life sciences
Cellular signal transduction
Cell physiology
Fluorescent probes
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Zhang, Jin (Molecular scientist), editor.
Ni, Qiang.
Newman, Robert H.
ISBN 9781627036221