Title C. elegans : methods and applications / edited by Kevin Strange

Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (xii, 292 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 351 Methods in molecular biology (Clifton, N.J.) ; v. 351 Contents An overview of C. elegans biology / Kevin Strange -- Comparative genomics in C. elegans, C. briggsae, and other Caenorhabditis species / Avril Coghlan, Jason E. Stajich, and Todd W. Harris -- WormBase: methods for data mining and comparative genomics / Todd W. Harris and Lincoln D. Stein -- C. elegans deletion mutant screening / Robert J. Barstead and Donald G. Moerman -- Insertional mutagenesis in C. elegans using the drosophila transposon Mos1: a methods for the rapid identification of mutated genes / Jean-Louis Bessereau -- Single nucleotide polymorphism mapping / M. Wayne Davis and Marc Hammarlund -- Creation of transgenic lines using microparticle bombardment methods / Vida Praitis -- Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA / Lisa Timmons -- Delivery methods for RNA interference in C. elegans / Lisa Timmons -- Functional genomic approaches in C. elegans / Todd Lamitina -- Assays for toxicity studies in C. elegans with Bt crystal proteins / Larry J. Bischof, Danielle L. Huffman, and Raffi V. Aroian -- Fluorescent reporter methods / Harald Hutter -- Electrophysiological analysis of neuronal and muscle function in C. elegans / Michael M. Francis and Andres Villu Maricq -- Sperm and oocyte isolation methods for biochemical and proteomic analysis / Michael A. Miller -- Preservation of C. elegans tissue via high-pressure freezing and freeze-substitution for ultrastructural analysis and immunocytochemistry / Robby M. Weimer -- Intracellular pH measurements in vivo using green fluorescent protein variants / Keith Nehrke -- Automated imaging of C. elegans behavior / Christopher J. Cronin, Zhaoyang Feng, and William R. Schafer -- Intracellular Ca2+ imaging in C. elegans / Rex A. Kerr and William R. Schafer -- In vitro culture of C. elegans somatic cells / Kevin Strange and Rebecca Morrison -- Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system / Rock Pulak Summary Molecular biology has driven a powerful reductionist, or "molecule-c- tric," approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of "naïve reductionism." Red- tionist methods will continue to be an essential element of all biological research efforts, but "naïve reductionism," the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2-5). The "systems" of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or "emergent" properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e Bibliography Includes bibliographical references and index Notes English Print version record Subject Caenorhabditis elegans. Caenorhabditis elegans -- Genetics Molecular biology. Life sciences. Rhabditida. Physical sciences. Physiology. Caenorhabditis elegans -- physiology Caenorhabditis elegans -- ultrastructure Biological Science Disciplines Caenorhabditis Rhabditoidea Natural Science Disciplines Disciplines and Occupations Rhabditida Nematoda Helminths Invertebrates Animals Eukaryota Organisms Physiology Caenorhabditis elegans Molecular Biology molecular biology. biological sciences. physical sciences. physiology. SCIENCE -- Life Sciences -- Molecular Biology. Rhabditida Physiology Physical sciences Life sciences Caenorhabditis elegans Caenorhabditis elegans -- Genetics Molecular biology Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Strange, Kevin, 1955- ISBN 1588295974 1597451517 9781597451512 9781588295972 128083241X 9781280832413 9786610832415 6610832412

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