| Description |
1 online resource (xii, 252 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1327 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 1327
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|
Springer protocols (Series)
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| Contents |
Library construction for mutation identification by whole-genome sequencing / Harold E. Smith -- Fundamentals of comparative genome analysis in caenorhabditis nematodes / Eric S. Haag and Cristel G. Thomas -- Genetic methods for cellular manipulations in C. elegans / Menachem Katz -- Fusion PCR method for expressing genetic tools in C. elegans / Yifat Eliezer and Alon Zaslaver -- Transposon-assisted genetic engineering with Mos1- mediated single-copy insertion (MosSCI) / Christian Frøkjær-Jensen -- Creating genome modifications in C. elegans using the CRISPR/Cas9 system / John A. Calarco and Ari E. Friedland -- Observing and quantifying fluorescent reporters / Michael Hendricks -- Microbial rhodopsin optogenetic tools : application for analyses of synaptic transmission and of neuronal network activity in behavior / Caspar Glock, Jatin Nagpal, and Alexander Gottschalk -- Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans / Nicholas F. Trojanowski and Christopher Fang-Yen -- High-pressure freeze and freeze substitution electron microscopy in C. elegans / Laura Manning and Janet Richmond -- Electron tomography methods for C. elegans / David H. Hall and William J. Rice -- Microfluidic devices for behavioral analysis, microscopy, and neuronal imaging in Caenorhabditis elegans / Ross C. Lagoy and Dirk R. Albrecht -- Tracking single C. elegans using a USB microscope on a motorized stage / Eviatar I. Yemini and André E.X. Brown -- Imaging system for C. elegans behavior / Matthew A. Churgin and Christopher Fang-Yen -- Method for obtaining large populations of synchronized Caenorhabditis elegans dauer larvae / Maria C. Ow and Sarah E. Hall -- Sampling and isolation of C. elegans from the natural habitat / Nausicaa Poullet and Christian Braendle -- Primer on prototyping / Dylan Lynch and David Biron -- Primer on quantitative modeling / Iulia Neagu and Erel Levine |
| Summary |
The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful 'Methods in Molecular Biology' series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Caenorhabditis elegans.
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Caenorhabditis elegans -- Genetics
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Molecular biology.
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Rhabditida.
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Caenorhabditis elegans
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Caenorhabditis
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Rhabditoidea
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Rhabditida
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Nematoda
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Helminths
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Invertebrates
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Animals
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Eukaryota
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Organisms
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Molecular Biology
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Rhabdiasoidea
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molecular biology.
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Animal reproduction.
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Animal pathology & diseases.
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Medical genetics.
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Science -- Life Sciences -- Zoology -- General.
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Medical -- Genetics.
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Rhabditida
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Caenorhabditis elegans
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Caenorhabditis elegans -- Genetics
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Molecular biology
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Biron, David.
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Haspel, Gal.
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| ISBN |
9781493928422 |
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1493928422 |
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1493928414 |
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9781493928415 |
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