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Book Cover
Author Davis, John M

Title Animal Cell Culture : Essential Methods
Published Hoboken : John Wiley & Sons, 2011


Description 1 online resource (709 pages)
Contents Cover; Title Page; Copyright; Dedication; Contributors; preface; Abbreviations; Protocols; 1 The Cell Culture Laboratory; 2: Sterilization; 3: Microscopy of Living Cells; 4: Basic Techniques and Media, the Maintenance of Cell Lines, and Safety; 5: Development and Optimization of Serum- and Protein-free Culture Media; 6: Cryopreservation and Banking of Cell Lines; 7: Primary Culture of Specific Cell Types and the Establishment of Cell Lines; 8: Cloning; 9: The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination; 10: Systems for Cell Culture Scale-up
11: Good Laboratory Practice in the Cell Culture LaboratoryColor Plate; Index
Summary This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Cell culture -- Technique
Tissue culture -- Technique
Cell culture -- Technique
Tissue culture -- Technique
Form Electronic book
LC no. 2010037008
ISBN 9780470669822