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Book Cover

Title Epidermal cells : methods and protocols / edited by Kursad Turksen
Published Totowa, N.J. : Humana Press, ©2005


Description 1 online resource (xv, 480 pages) : illustrations
Series Methods in molecular biology ; v. 289
Methods in molecular biology (Clifton, N.J.) ; v. 289
Contents Primary mouse keratinocyte culture / Annalisa Pirrone, Barbara Hager, and Philip Fleckman -- Serial cultivation of primary adult murine keratinocytes / Richard P. Redvers and Pritinder Kaur -- Keratinocyte culture in the absence of substrate attachment / Monika Jost and Ulrich Rodeck -- Application of genetically modified feeder cells for culture of keratinocytes / Takashi Kameda and Toshihiro Sugiyama -- Organ culture of developing mouse skin and its application for molecular mechanistic studies of morphogenesis / Mariko Kashiwaga and Nam-ho Huh -- Experimental models to analyze differentiation functions of cultured keratinocytes in vitro and in vivo / Nicole Maas-Szabowski, Norbert E. Fusenig, and Hans-Jürgen Stark -- In vitro fabrication of engineered human skin / Alexander Margulis, Weitian Zhang and Jonathan A. Garlick -- In vivo labeling and analysis of epidermal stem cells / Wei-yang Wu and Rebecca J. Morris -- Method for the harvest and assay of in vitro clonogenic keratinocytes stem cells from mice / Wei-yang Wu and Rebecca J. Morris -- FACS enrichment of human keratinocyte stem cells / Amy Li and Pritinder Kaur -- Isolation, characterization, and culture of epithelial stem cells / Jackie R. Bickenbach -- Keratin 19 as a stem cell marker in vivo and in vitro / Danielle Larouche [and others] -- Immunolocalization in the epidermis / Tammy-Claire Troy [and others] -- Epidermal cell analysis by RT-PCR / Tammy-Claire Troy [and others] -- Whole-mount assays for gene induction and barrier formation in the developing epidermis / Carolyn Byrne and Matthew J. Hardman -- Analysis of early epidermal development in zebrafish / Ashley E. Webb and David Kimelman -- Analysis of E2F factors during epidermal differentiation / Wing Y. Chang and Lina Dagnino -- Analysis of HOX homeodomain proteins and gene transcripts
Apoptosis in the epidermis / Kiyofumi Yamanishi, Chun-Shen Shen and Hitoshi Mizutani -- Fate of desmosomal proteins in apoptotic epidermal cells / J. Weiske and Otmar Huber -- Analysis of connexin 43 expression on keratinocytes using flow cytometry / Maja Matic [and others] -- MMP-9 and TIMP-1 assays in keratinocyte cultures / Takashi Kobayashi -- Characterization of epithelial cells in the hair follicle with S100 proteins / Kenji Kizawa and Mayumi Ito -- Immunoelectron microscopic analysis of cornified cell envelopes and antigen retrieval / Akemi Ishida-Yamamoto -- Cell kinetic analysis in artificial skin using immunochemical methods / Andrea Casasco [and others] -- Proliferation, differentiation, and inflammation in normal and hyperproliferative skin using multiparameter flow cytometry / Piet E.J. van Erp -- Fluorimetric DNA assay of cell number / William R. Otto -- Keratinocyte transient transfections / Anthony M. Flores and Brian J. Aneskievich -- Tetracycline-regulated gene expression in epidermal keratinocytes / Richard B. Presland and Philip Fleckman -- Gene targeting by oligonucleotides in keratinocytes / Olga Igoucheva and Kyonggeun Yoon -- Promoter analysis in the human SPRR gene family / David F. Fischer and Claude Backendorf -- Stable integration of large PAC contructs in keratinocytes / Sarah H. Williams and Alain Hovnanian -- Targeted somatic mutagenesis in the mouse epidermis / Daniel Metzger, Mei Li, and Pierre Chambon -- Methods to study protein/protein interactions / Jin-jun Meng [and others] -- Isolation of recombinant phage displayed antibodies recognizing skin keratinocytes / Kim Bak Jensen and Peter Kristensen -- Analysis of tissue-specific DNA methylation during development / Jun Ohgane, Naka Hattori and Kunio Shiota -- Serial analysis of gene expression in human keratinocytes and the epidermis / Bastiaan J.H. Jansen [and others] -- Methods for gene expression profiling in dermatology research using dermarray nylon filter DNA microarrays / Richard L. Davis, Jr. [and others] -- Two-photon fluorescence imaging and reactive oxygen species detection within the epidermis / Kerry M. Hanson and Robert M. Clegg -- In vivo transplantation of engineered human skin / Shari Greenberg, Alexander Margulis and Jonathan A. Garlick -- Epidermis targeted gene transfer using in vivo electroporation / Hiroki Maruyama, Jun-ichi Miyazaki, and Fumitake Gejyo -- Gene and stem cell therapy of the hair follicle / Robert M. Hoffman
Summary In recent years our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including wound healing and transplantation. In Epidermal Cells: Methods and Protocols, a panel of experienced basic and clinical researchers describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Epidermis -- Physiology -- Research -- Methodology
Epidermis -- Cytology -- Research -- Methodology
Cytological Techniques -- methods
Genetic Engineering -- methods
Keratinocytes -- cytology
Organ Culture Techniques -- methods
Epidermal Cells
Cell Culture Techniques -- methods
MEDICAL -- Research.
Epidermis (dierkunde)
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Turksen, Kursad
ISBN 1588292673