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Book Cover

Title Basic cell culture protocols / edited by Cheryl D. Helgason, Cindy L. Miller
Edition 4th edition
Published New York : Humana Press : Springer, ©2013


Description 1 online resource (xiv, 550 pages) : illustrations (some color)
Series Methods in molecular biology ; v. 946
Methods in molecular biology (Clifton, N.J.) ; volume 946
Contents Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- STR DNA typing of human cell lines : detection of intra- and interspecies cross-contamination / Wilhelm G. Dirks and Hans G. Drexler -- Classical and molecular cytogenetic analysis / Roderick A.F. MacLeod and Hans G. Drexler -- Fluorescent in situ hybridization of DNA probes in the interphase and metaphase stages of the cell cycle / Linda A. Cannizzaro -- Development of T lymphocytes in fetal thymus organ culture / Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama -- Generation, isolation, and engraftment of in vitro-derived human T cell progenitors / Geneve Awong and Juan Carlos Zuniga-Pflucker -- In vitro generation of human T regulatory cells : generation, culture, and analysis of FOXP3-transduced T cells / Alicia N. McMurchy and Megan K. Levings -- Simultaneous cloning and selection of hybridomas and transfected cell lines in semisolid media / Bert Wognum and Tracy Lee -- Isolation and characterization of mouse side population cells / Aysegul V. Ergen [and others] -- Stem cell identification by DyeCycle violet side population analysis / William G. Telford -- Isolation and characterization of cancer stem cells in vitro / Craig Gedye and Laurie Ailles -- Ex vivo differentiation of cord blood stem cells into megakaryocytes and platelets / Nicolas Pineault [and others] -- Generation and characterization of murine alternatively activated macrophages / Shelley B. Weisser [and others] -- Human long-term culture initiating cell assay / Min Liu, Cindy L. Miller, and Connie J. Eaves -- Long-term culture-initiating cell assay for mouse cells / Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves -- Colony forming cell assays for human hematopoietic progenitor cells / Bert Wognum [and others] -- Studying leukocyte recruitment under flow conditions / Sean A. Parsons [and others] -- Generation and establishment of murine adherent cell lines / Rouzanna Istvanffy and Robert A.J. Oostendorp -- Isolation, enumeration, and expansion of human mesenchymal stem cells in culture / Ravenska Wagey and Brenton Short -- Isolation and culture of mesenchymal stem cells from mouse compact bone / Brenton Short and Ravenska Wagey -- Generation of a pool of human platelet lysate and efficient use in cell culture / Katharina Schallmoser and Dirk Strunk -- In vitro methods to culture primary human breast epithelial cells / Afshin Raouf and Yu Jia Sun -- Human prostate epithelial cell cultures / Johng S. Rhim -- Enzymatic dissociation, flow cytometric analysis, and culture of normal mouse mammary tissue / Michael Prater [and others] -- Isolation and characterization of human hair follicle epithelial cells / Keita Inoue and Kotaro Yoshimura -- Cocultivation of human oral keratinocytes and human osteoblast-like cells / Ricarda Glaum and Margit Wiedmann-Al-Ahmad -- Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Paul Keire [and others] -- Hepatic differentiation of embryonic stem cells by murine fetal liver mesenchymal cells / Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai -- Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system / Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds -- Feeder-independent culture systems for human pluripotent stem cells / Jennifer Moody -- Formation of embryoid bodies from human pluripotent stem cells using AggreWell plates / Jennifer Antonchuk -- Techniques in embryoid body formation from human pluripotent stem cells / Nirupama K. Shevde and Amber A. Mael
Summary At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular Biology, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success
Analysis celbiologie
cellular biology
cell culture
Bibliography Includes bibliographical references and index
Notes English
Subject Cell culture -- Laboratory manuals
Tissue culture -- Laboratory manuals
Cell Culture Techniques -- methods
Tissue Culture -- methods
Cell culture
Tissue culture
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Helgason, Cheryl D.
Miller, Cindy L.
LC no. 2012950590
ISBN 9781627031288