Title Basic cell culture protocols / edited by Jeffrey W. Pollard and John M. Walker

Edition 2nd ed Published Totowa, N.J. : Humana Press, ©1997

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Description 1 online resource (xiv, 489 pages) : illustrations Series Methods in molecular biology ; v. 75 Methods in molecular biology (Clifton, N.J.) ; v. 75 Contents Basic cell culture -- Establishment, maintenance, and cloning of human dermal fibroblasts -- Aging of cultured human skin fibroblasts -- Ex vivo maintenance of differentiated mammalian cells -- Scale-up of suspension and anchorage-dependent animal cells -- Hollow-fiber cell culture -- Separation and maintenance of primary T- and B-lymphocytes -- Human pilosebaceous culture -- Keratinocyte culture -- Tissue culture of skeletal muscle -- Isolation of rat liver hepatocytes -- Primary kidney cells -- Human thyroid epithelial cells -- Derivation and maintenance of embryonic stem cell cultures -- Culture of cells from human tumors of the nervous system on an extracellular matrix derived from bovine corneal endothelial cells -- Culturing primitive hemopoietic cells -- Collagen gel culture of the human hematopoietic progenitors cFU-GM, CFU-E, and BFU-E -- Long-term B-lymphoid cultures from murine bone marrow establishment and coloning by using stomal cell line AC 6.21 -- Human long-term bone marrow culture -- In vitro clonal culture of human hematopoietic progenitor cells -- High proliferative potential colony-forming cells -- Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors -- Flow sorting for isolating CFU-E -- Murine bone marrow-derived macrophages -- Routine testing of cell cultures and their products for mycoplasma contamination -- Fluorescent in situ hybridization (FISH) for DNA probes in the interphase and metaphases stages of the cell cycle -- Cytogenetic techniques for human leukemias -- Visualization of cell replication using antibody to proliferating cell nuclear antigen -- Proliferation of murine myoblasts as measured by BrdUrd incorporation -- Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method -- nRNA in situ hybridization to in vitro cultured cells -- Transmission and scanning electron microscope preparation of whole cultured cells -- Adhesion molecules on isolated and cultured microvascular endothelial cells demonstrated by immunofluorescence and immune electron microscopy -- Producation of heterologous proteins using the baculovirus/insect expression system -- Transfection and transformation of human thyroid epithelial cells -- Analyzing chemotaxis using the Dunn direct-viewing chamber -- Computer-assisted analysis of single-cell behavior Summary Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its distinguished contributors-often the perfectors of the detailed techniques being described-provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Basic Cell Culture Protocols, 2nd ed. will surely follow in the footsteps of the first edition and, in the words of the reviewers, prove a still more "clear, easy-to-use well-sourced, and precise" work that belongs "in every cell culture laboratory." Analysis celkweek cell culture weefselkweek tissue culture biologische technieken biological techniques methodologie methodology kweektechnieken culture techniques protocollen protocols Biological Techniques Biologische technieken Notes Previous edition published as: Animal cell culture, 1990 Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL SUBJECT Animal 2017 Bildband gnd Subject Cell culture -- Technique Tissue culture -- Technique Cultures (Biology) Cell Culture Techniques -- methods Culture Techniques Culture Techniques -- methods Cell Culture Techniques Cultures (Biology) Cell culture -- Technique Tissue culture -- Technique Cellules cultivées. Biologie moléculaire -- Méthodes. Zellkultur Aufsatzsammlung Celkweek. Wetenschappelijke technieken. Cellules -- Cultures et milieux de culture. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Pollard, Jeffrey W. Walker, John M., 1948- ISBN 9781592595617 1592595618 1280836806 9781280836800 9786610836802 6610836809

Other Titles Animal cell culture

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