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Title Cell cycle checkpoint control protocols / edited by Howard B. Lieberman
Published Totowa, N.J. : Humana Press, ©2004


Description 1 online resource (xvi, 372 pages) : illustrations
Series Methods in molecular biology ; 241
Methods in molecular biology (Clifton, N.J.) ; v. 241
Contents pt. 1. Induction and detection of changes in cell cycle progression -- Methods to induce cell cycle checkpoints / Howard B. Lieberman -- Methods for synchronizing mammalian cells / Michael H. Fox -- Enrichment of cells in different phases of the cell cycle by centrifugal elutriation / Tej K. Pandita -- Analysis of the mammalian cell cycle by flow cytometry / Haiying Hang -- Methods for detecting cells in S phase / Wei-Hsin Sun -- Yeast cell synchronization / Audra Day -- Analysis of the budding yeast Saccharomyces cerevisiae cell cycle by morphological criteria and flow cytometry / Hong Zhang -- Analysis of the fission yeast Schizosaccharomyces pombe cell cycle / Eliana B. Gómez -- pt. 2. Analysis of genes involved in checkpoint control -- Strategies to isolate evolutionarily conserved cell cycle regulatory genes / Scott Davey -- Microarray approaches for analysis of cell cycle regulatory genes / Sally A. Amundson -- Using the yeast genome-wide gene-deletion collection for systematic genetic screens / Jian Zhang -- Gene targeting in cultured human cells / Todd A. Waldman -- Use of in vivo gap repair for isolation of mutant alleles of a checkpoint gene / Migdalisel Colón -- In vitro mutagenesis to define functional domains / Jian Qin -- Use of gene overexpression to assess function in cell cycle control / Erik K. Flemington -- Histone acetylation/deacetylation as a regulator of cell cycle gene expression / Chenguang Wang -- pt. 3. Analysis of proteins involved in checkpoint control -- Cataloging proteins in cell cycle control / Kazimierz O. Wrzeszczynski -- Multidimensional proteomic analysis of proteolytic pathways involved in cell cycle control / Michael W. Schmidt -- Purification and identification of protein complexes that control the cell cycle / Matthew A. Burtelow -- Xenopus cell-free extracts to study DNA damage checkpoints / Vincenzo Costanzo -- Protein-protein interactions / Graziella Pedrazzi -- Detection of kinase and phosphatase activities /Sean M. Post -- Monitoring changes in the subcellular location of proteins in S. cerevisiae / Diego Rua -- pt. 4. Chromosomes and the cell cycle -- Chromosomal changes and cell cycle checkpoints in mammalian cells / Charles R. Geard -- Detecting the influence of cell cycle regulatory proteins on human telomeres / Tej K. Pandita -- Monitoring spindle assembly and disassembly in yeast by indirect immunofluorescence / Rita K. Miller
Summary In recent years, cell cycle checkpoints, cellular mechanisms that control the cell cycle and ensure genomic stability, have emerged as significant factors in carcinogenesis and in cancer cells. In Cell Cycle Checkpoint Control Protocols, leading investigators present their best methodologies to probe the mechanisms underlying cell cycle regulation and checkpoint control. Using mammalian, yeast, and frog systems, these experts describe readily reproducible methods to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and characterize chromosomal status as a function of cell cycle phase and progression. Each fully tested technique includes step-by-step instructions written by an investigator who performs it frequently, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Taken as a whole, the collection describes the major methodologies used by researchers in the field. Cutting-edge and highly practical, Cell Cycle Checkpoint Control Protocols provides an extensive array of detailed protocols by which both experienced and novice investigators may successfully illuminate questions concerning cell cycle control
Bibliography Includes bibliographical references
Notes English
Print version record
In Springer Protocols
Subject Cell cycle -- Laboratory manuals
Cell Cycle -- physiology
Genes, cdc
Yeasts -- physiology
SCIENCE -- Life Sciences -- Developmental Biology.
Cell cycle
Wetenschappelijke technieken.
Genre/Form Handbooks and manuals
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Lieberman, Howard B.
ISBN 1592596460