| Description |
1 online resource (xii, 228 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1064-3745 ; volume 1545 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1545. 1064-3745
|
| Contents |
Methods to analyze EVs / Bernd Giebel and Clemens Helmbrecht -- Tunable resistive pulse sensing for the characterization of extracellular vesicles / Sybren L.N. Maas, Marike L.D. Broekman, and Jeroen de Vrij -- Immuno-characterization of exosomes using nanoparticle tracking analysis / Kym McNicholas and Michael Z. Michael -- Imaging and quantification of extracellular vesicles by transmission electron microscopy / Romain Linares, Sisareuth Tan, Céline Gounou, and Alain R. Brisson -- Quantitative analysis of exosomal miRNA via qPCR and digital PCR / Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill -- Small RNA library construction for exosomal RNA from biological samples for the Ion Torrent PGM and Ion S5TM system / Lesley Cheng and Andrew F. Hill -- Protocol for isolation and proteomic characterization of distinct extracellular vesicle subtypes by sequential centrifugal ultrafiltration / Rong Xu, Richard J. Simpson, and David W. Greening -- Multiplexed phenotyping of small extracellular vesicles using protein microarray (EV Array) / Rikke Bæk and Malene Møller Jørgensen -- Purification and analysis of exosomes released by mature cortical neurons following synaptic activation / Karine Laulagnier, Charlotte Javalet, Fiona J. Hemming, and Rémy Sadoul -- Method for isolation of extracellular vesicles and characterization of exosomes from brain extracellular space / Rocío Perez-Gonzalez, Sebastien A. Gauthier, Asok Kumar, Mitsuo Saito, Mariko Saito, and Efrat Levy -- Isolation of exosomes and microvesicles from cell culture systems to study prion transmission / Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graça Raposo, and Didier Vilette -- Isolation of platelet-derived extracellular vesicles / Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland, and Pia Siljander -- Bioinformatics tools for extracellular vesicles research / Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan -- Preparation and isolation of siRNA-loaded extracellular vesicles / Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E.L. Andaloussi, and Matthew J.A. Wood -- Interaction of extracellular vesicles with endothelial cells under physiological flow conditions / Susan M. van Dommelen, Margaret Fish, Arjan D. Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, and Pieter Vader -- Flow cytometric analysis of extracellular vesicles / Aizea Morales-Kastresana and Jennifer C. Jones |
| Summary |
This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed December 12, 2016) |
| Subject |
Extracellular space -- Laboratory manuals
|
|
Exosomes
|
|
Cell-Derived Microparticles
|
|
Laboratory Manuals
|
|
Extracellular space
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hill, Andrew F. (Andrew Francis), editor.
|
| ISBN |
9781493967285 |
|
1493967282 |
|
1493967266 |
|
9781493967261 |
|
