| Description |
1 online resource (xi, 231 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; 1242 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1242
|
| Contents |
Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells / Maja G. Rydahl [and others] -- Using chemical genomics to study cell wall formation and cell growth in Arabidopsis thaliana and Penium margaritaceum / N. Worden [and others] -- Optimized method for growing in vitro Arabidopsis thaliana pollen tubes / Cecilia Borassi [and others] -- Imaging of calcium dynamics in pollen tube cytoplasm / María Laura Barberini and Jorge Muschietti -- Live imaging of root hairs / Silvia M. Velasquez, Jose R. Dinneny, and José M. Estevez -- Improved ROS measurement in root hair cells / Paola Silvina Denita Juárez, Silvina Mangano, and José M. Estevez -- Root hair assay to expedite cell death research / Joanna Kacprzyk and Paul F. McCabe -- Vacuolar staining methods in plant cells / David Scheuring [and others] -- Live cell imaging of FM4-64, a tool for tracing the endocytic pathways in Arabidopsis root cells / Adeline Rigal, Siamsa M. Doyle, and Stéphanie Robert -- Salt-stress regulation of root system growth and architecture in Arabidopsis seedlings / Lina Duan, Jose Sebastian, and Jose R. Dinneny -- Quantification of fluorescent reporters in plant cells / Michael Pound, Andrew P. French, and Darren M. Wells -- Live cell imaging of the cytoskeleton and cell wall enzymes in plant cells / Arun Sampathkumar and Raymond Wightman -- Using the split-ubiquitin yeast two-hybrid system to test protein-protein interactions of transmembrane proteins / Logan Bashline and Ying Gu -- Activation tag screening for cell expansion genes in Arabidopsis thaliana / Chaowen Xiao and Charles T. Anderson -- BiFC for protein-protein interactions and protein topology : discussing an integrative approach for an old technique / Giovanni Stefano, Luciana Renna, and Federica Brandizzi -- N-Glycosylation and plant cell growth / Christiane Veit, Ulrike Vavra, and Richard Strasser -- Peptide separation methodologies for in-depth proteomics / Sajad Majeed Zargar [and others] -- Structural and mechanical characterization of growing Arabidopsis plant cell walls / Friederike Saxe, Ingo Burgert, and Michaela Eder |
| Summary |
This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists |
| Analysis |
plantencelbiologie |
|
plant cell biology |
|
cellen |
|
cells |
|
plantenfysiologie |
|
plant physiology |
|
planten |
|
plants |
|
Plant Cell Biology |
|
Plantencelbiologie |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Plant cells and tissues -- Laboratory manuals
|
|
Plant physiology.
|
|
Plant Cells
|
|
Cell Enlargement
|
|
Cell Size
|
|
Plant Physiological Phenomena
|
|
Plant physiology.
|
|
Botany & plant sciences.
|
|
Science -- Life Sciences -- Botany.
|
|
Plant physiology
|
|
Plant cells and tissues
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Estevez, José M.
|
| ISBN |
9781493919024 |
|
1493919024 |
|
