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Title Cell cycle synchronization : methods and protocols / edited by Gaspar Banfalvi
Published New York : Humana Press, 2011


Description 1 online resource : illustrations
Series Springer protocols
Methods in molecular biology, 1064-3745 ; v. 761
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; volume 761. 1064-3745
Contents 1. Overview of cell synchronization / Gaspar Banfalvi -- 2. Synchronization of mammalian cells and nuclei by centrifugal elutriatio / Gaspar Banfalvi -- 3. Cytofluorometric purification of diploid and tetraploid cancer cells / Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer -- 4. Large-scale mitotic cell synchronization / Kalyan Dulla and Anna Santamaria -- 5. Synchronization of mammalian cell cultures by serum deprivation / Thomas J. Langan and Richard C. Chou -- 6. Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry / Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka -- 7. Chromosome formation during fertilization in eggs of the teleost oryzias latipes / Takashi Iwamatsu -- 8. Specific cell cycle synchronization with butyrate and cell cycle analysis / Congjun Li -- 9. Analysis of nuclear uracil-DNA glycosylase (nUDG) turnover during the cell cycle / Jennifer A. Fischer and Salvatore Caradonna -- 10. Synchronization of HeLa cells / Hoi Tang Ma and Randy Y.C. Poon -- 11. Synchronization of Bacillus subtilis cells by spore germination and outgrowth / Gasper Banfalvi -- 12. Synchronization of yeast / Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider -- 13. Synchronization of pathogenic protozoans / Staffan Svärd and Karin Troell -- 14. Synchronization of in vitro maturation porcine oocytes / Tamas Somfai and Yuji Hirao -- 15. Synchronization of medicago sativa cell suspension culture / Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth -- 16. Cell cycle synchronization for the purpose of somatic cell nuclear transfer (SCNT) / Yoel Shufaro and Benjamin E. Reubinoff -- 17. Ex vivo expansion of haematopoietic stem cells to improve engraftment in stem cell transplantation / Kap-Hyoun Ko, Robert Nordon, Tracey A. O'Brien, Geoff Symonds, and Alla Dolnikov -- 18. Flow cytometry developments and perspectives in clinical studies: examples in ICU patients / Fabienne Venet, Caroline Guignant, and Guillaume Monneret -- 19. Molecular network dynamics of cell cycle control: transitions to start and finish / Attila Csikász-Nagy, Alida Palmisano, and Judit Zámbprszky
Summary To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the "arrest-and-release" approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so
Analysis Cell Biology
Cell Cycle Analysis
Life Sciences
cellular biology
cell cycle
Cellular Biology
Bibliography Includes bibliographical references
Notes English
Subject Life sciences.
Cell culture -- Laboratory manuals
Cell cycle -- Regulation -- Laboratory manuals
Cell cycle.
Cell Culture Techniques
Cell Cycle
Biological Science Disciplines
Cell Biology
biological sciences.
Cell cycle
Cell culture
Cell cycle -- Regulation
Life sciences
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Bánfalvi, Gáspár.
LC no. 2011931108
ISBN 9781617791826