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Title 3D cell culture : methods and protocols / edited by John W. Haycock
Published New York, NY : Humana Press, 2011


Description 1 online resource (xi, 343 pages) : illustrations
Series Springer protocols
Methods in molecular biology, 1940-6029 ; 695
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 695. 1064-3745
Contents 3d cell culture: A review of current approaches and techniques / John W. Haycock -- Scaffolds for tissue engineering and 3d cell culture / Eleonora Carletti ; Antonella Motta ; Claudio Migliaresi -- Tracking nanoparticles in three-dimensional tissue-engineered models using confocal laser scanning microscopy / Vanessa Hearnden ; Sheila MacNeil ; Giuseppe Battaglia -- Using immuno-scanning electron microscopy for the observation of focal adhesion-substratum interactions at the nano- and microscale in S-phase cells / Manus J.P. Biggs ; R. Geoff Richards ; Matthew J. Dalby -- 3d sample preparation for orthopaedic tissue engineering bioreactors / Sarah H. Cartmell ; Sarah Rathbone ; Gemma Jones ; L. Araida Hidalgo-Bastida -- Quantification of mRNA using real-time PCR and western blot analysis of MAPK events in chondrocyte/agarose constructs / David A. Lee ; June Brand ; Donald Salter ; Oto-Ola Akanji ; Tina T. Chowdbury -- Genetic modification of chondrocytes using viral vectors / Teresa Coughlan ; Aileen Crawford ; Paul Hatton ; Michael Barker -- Stem cell and neuron co-cultures for the study of nerve regeneration / Paul J. Kingham ; Cristina Mantovani ; Giorgio Terenghi -- Production of tissue-engineered skin and oral mucosa for clinical and experimental use / Sheila MacNeil ; Joanna Shepherd ; Louise Smith -- Three-dimensional alignment of Schwann cells using hydrolysable microfiber scaffolds: Strategies for peripheral nerve repair / Celia Murray-Dunning ; Sally L. McArthur ; Tao Sun ; Rob McKean ; Anthony J. Ryan ; John W. Haycock -- Encapsulation of human articular chondrocytes into 3d hydrogel: Phenotype and genotype characterization / Rui C. Pereira ; Chaira Gentili ; Ranieri Cancedda ; Helena S. Azevedo ; Rui L. Reis -- Micro-structured materials and mechanical cues in 3d collagen gels / James B. Phillips ; Robert Brown -- Organotypic and 3d reconstructed cultures of the human bladder and urinary tract / Claire L. Varley ; Jennifer Southgate -- Ex vivo organ culture of human hair follicles: A model epithelial-neuroectodermal-mesenchymal interaction system / Desmond J. Tobin -- Human endothelial and osteoblast co-cultures on 3d biomaterials / Ronald E. Unger ; Sven Halstenberg ; Anne Sartoris ; C. James Kirkpatrick -- Assessment of nanomaterials cytotoxicity and internalization / Noha M. Zaki ; Nicola Tirelli -- Practical aspects of OCT imaging in tissue engineering / Stephen J. Matcher -- Osteogenic differentiation of embryonic stem cells in 2d and 3d culture / Lee Buttery ; Robert Bielby ; Daniel Howard ; Kevin Shakesheff -- 3d structuring of biocompatible and biodegradable polymers via stereolithography / Andrew A. Gill ; Frederik Claeysseus -- Alvetex: Polystyrene scaffold technology for routine three dimensional cell culture / Eleanor Knight ; Bridgid Murray ; Ross Carnachan ; Stefan Przyborski
Summary Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format
Analysis celkweek
cell culture
cellular biology
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Cell culture -- Technique
Cell Culture Techniques -- methods
Cell Culture Techniques
Cell culture -- Technique
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Haycock, John W
ISBN 9781607619840