| Description |
1 online resource (xvi, 452 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 1612 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1612. 1064-3745
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| Contents |
3D cell culture : an introduction / Zuzana Koledova -- Preparation of decellularized biological scaffolds for 3D cell culture / Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest -- 3D cell culture in interpenetrating networks of alginate and rBM matrix / Katrina Wisdom and Ovijit Chaudhuri -- Hydrogel-based in vitro models of tumor angiogenesis / Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner -- Generation of induced pluripotent stem cells in defined three-dimensional hydrogels / Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf -- Calcium phosphate foams : potential scaffolds for bone tissue modeling in three dimensions / Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra -- Establishment of 3D intestinal organoid cultures from intestinal stem cells / Shinya Sugimoto and Toshiro Sato -- 3D coculture of mammary organoids with fibrospheres : a model for studying epithelial-stromal interactions during mammary branching morphogenesis / Zuzana Koledova -- Organotypic 3D assay for primary human mammary epithelial cells that recapitulates branching morphogenesis / Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel -- 3D primary Culture model to study human mammary development / Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta -- Lungosphere assay : 3D culture of lung epithelial stem/progenitor cells / Anas Rabata, Ales Hampl, and Zuzana Koledova -- 3D hanging drop culture to establish prostate cancer organoids / Theresa Eder and Iris E. Eder -- 3D-dynamic culture models of multiple myeloma / Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero -- Preparation of a three-dimensional full thickness skin equivalent / Christian Reuter, Heike Walles, and Florian Groeber -- Analysis of breast cancer cell invasion using an organotypic culture system / Romana E. Ranftl and Fernando Calvo -- 3D coculture model of the brain parenchyma-metastasis interface of brain metastasis / Raquel Blazquez and Tobias Pukrop -- 3D neural culture in dual hydrogel systems / J. Lowry Curley and Michael J. Moore -- 3D cell culture in micropatterned hydrogels prepared by photomask, microneedle, or soft lithography techniques / Seyedsina Moeinzadeh and Esmaiel Jabbari -- 3D stem cell niche engineering via two-photon laser polymerization / Michele M. Nava, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, and Manuela T. Raimondi -- Microfluidic-based generation of 3D collagen spheres to investigate multicellular spheroid invasion / Fabien Bertillot, Youmna Attieh, Morgan Delarue, Basile G. Gurchenkov, Stephanie Descroix, Danijela Matic Vignjevic, and Davide Ferraro -- High-throughput cancer cell sphere formation for 3D cell culture / Yu-Chih Chen and Euisik Yoon -- High-throughput 3D tumor culture in a recyclable microfluidic platform / Wenming Liu and Jinyi Wang -- High-throughput microfluidic platform for 3D cultures of mesenchymal stem cells / Paola Occhetta, Roberta Visone, and Marco Rasponi -- 3D anastomosed microvascular network model with living capillary networks and endothelial cell-lined microfluidic channels / Xiaolin Wang, Duc T.T. Phan, Steven C. George, Christopher C.W. Hughes, and Abraham P. Lee -- Human lung small airway-on-a-Chip protocol / Kambez H. Benam, Marc Mazur, Youngjae Choe, Thomas C. Ferrante, Richard Novak, and Donald E. Ingber -- Microfluidic bioprinting of heterogeneous 3D tissue constructs / Cristina Colosi, Marco Costantini, Andrea Barbetta, and Mariella Dentini -- Bioprinting of 3D tissue models using decellularized extracellular matrix bioink / Falguni Pati and Dong-Woo Cho -- Bioprinting cartilage tissue from mesenchymal stem cells and PEG hydrogel / Guifang Gao, Karen Hubbell, Arndt F. Schilling, Guohao Dai, and Xiaofeng Cui -- Real-time cell cycle imaging in a 3D cell culture model of melanoma / Loredana Spoerri, Kimberley A. Beaumont, Andrea Anfosso, and Nikolas K. Haass -- Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy / Josef Jaros, Michal Petrov, Marketa Tesarova, and Ales Hampl -- Quantitative phenotypic image analysis of three-dimensional organotypic cultures / Malin Åkerfelt, Mervi Toriseva, and Matthias Nees |
| Summary |
This title provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed June 27, 2017) |
| Subject |
Cell culture -- Technique
|
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Cell Culture Techniques -- methods
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Cell Culture Techniques
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Cellular biology (cytology)
|
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Science -- Life Sciences -- Biology -- Molecular Biology.
|
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Cell culture -- Technique
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| Genre/Form |
Laboratory manuals.
|
| Form |
Electronic book
|
| Author |
Koledova, Zuzang, editor
|
| ISBN |
9781493970216 |
|
1493970216 |
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1493970194 |
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9781493970193 |
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