Title Animal cell biotechnology : methods and protocols / edited by Ralf Pörtner

Edition 2nd ed Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (xv, 512 pages) : illustrations (some color) Series Methods in biotechnology, 1940-607X ; 24 Methods in biotechnology ; 24. 1940-6061 Contents pt. 1. Basic techniques for establishing production cell lines -- Transduction technologies / D.A. Fluri and M. Fussenegger -- Cells for gene therapy and vector production / C. Delenda [and others] -- Technology and production of murine monoclonal and recombinant antibodies and antibody fragments / A. Dorn-Beineke, S. Nittka and M. Neumaier -- DNA fingerprinting and characterization of animal cell lines / G.N. Stacey, E. Byrne and J.R. Hawkins -- pt. 2. Basic cultivation techniques -- Microcarrier cell culture technology / G. Blüml -- Cell encapsulation / H. Hübner -- Tools for high-throughput medium and process optimization / M. Jordan and N. Jenkins -- pt. 3. Cell characterization and analysis -- Cell counting and viability measurements / M. Butler and M. Spearman -- Monitoring of growth, physiology and productivity of animal cells by flow cytometry / S. Carroll, M. Naeiri and M. Al-Rubeai -- Nuclear magnetic resonance methods for monitoring cell growth and metabolism in intensive bioreactors / A.A. Neves and K.M. Brindle -- Methods for off-line analysis of nutrients and products in mammalian cell culture / H. Büntemeyer -- Application of stoichiometric and kinetic analyses to characterize cell growth and product formation / D. Adams, R. Korke, and W.-S. Hu -- Measurement of apoptosis in cell culture / A. Ishaque1 and M. Al-Rubeai -- Metabolic flux estimation in mammalian cell cultures / C.T. Goudar [and others] -- pt. 4. Special cultivation techniques -- Disposable bioreactors for inoculum production and protein expression / R. Eibl and D. Eibl -- Hollow fiber cell culture / J.M. Davis -- Cultivation of mammalian cells in fixed bed reactors / ̦R. Pörtner and O.B.J. Platas Barradas -- Configuration of bioreactors / D.E. Martens and E.J. van den End -- pt. 5. Downstream techniques -- Membrane filtration in animal cell culture / P. Czermak, D. Nehring and R. Wickramasinghe -- Chromatographic techniques in the downstream processing of (recombinant) proteins / R. Freitag -- pt. 6. Special applications -- Vaccine production : State of the art and future needs in upstream processing / Y. Genzel and U. Reichl -- Retrovirus production and characterization / P.E. Cruz [and others] -- Insect cell cultivation and generation of recombinant baculovirus particles for recombinant protein production / S. Geisse Summary Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes Analysis cellijnen cell lines dierlijke weefsels animal tissues biotechnologie biotechnology celkweek cell culture laboratoriummethoden laboratory methods technieken techniques farmaceutische producten pharmaceutical products celbiologie cellular biology protocollen protocols Biotechnology (General) Cellular Biology Biotechnologie (algemeen) Celbiologie Bibliography Includes bibliographical references and index Notes Print version record In Buchausg. u.d.T.: Animal cell biotechnology Subject Animal cell biotechnology. Cell culture. Biotechnology -- methods Cells, Cultured Cell Culture Techniques -- veterinary SCIENCE -- Biotechnology. Animal cell biotechnology. Cell culture. Animal cell biotechnology Cell culture Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Pörtner, Ralf. ISBN 9781592594863 1592594867 9781597453998 1597453994 128097155X 9781280971556 9786610971558 6610971552 9781627039635 1627039635

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