| Description |
1 online resource (xi, 337 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 348 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 348
|
| Contents |
Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di Berardino -- In vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in non-human primates / Shoukhrat M. Mitalipov and Don P. Wolf |
|
Cloning of exotic/endangered species : desert bighorn sheep / James "Buck" Williams [and others] -- Handmade somatic cell cloning in cattle / Gábor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu -- Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells / A. John Clark, Sarah Burl, and Chris Denning -- Transgenesis using nuclear transfer in goats / Anthoula Lazaris [and others] -- Nuclear reprogramming : an overview / Takashi Tada -- Expression of imprinted genes in cloned mice / Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura -- Nuclear remodeling assay in xenopus egg extract / Koichi Gonda and Nobuaki Kikyo -- In vitro reprogramming of nuclei and cells / Anne-Mari Håkelien [and others] -- Techniques for nuclear transfer to mouse embryonic stem cells / Danielè Pralong and Paul J. Verma -- Gene expression and methylation patterns in cloned embryos / Christine Wrenzycki [and others] -- Are there any normal clones? / Ian Wilmut -- Future and applications of cloning / Alan O. Trounson |
| Summary |
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Cell nuclei -- Transplantation.
|
|
Gene therapy.
|
|
Transgenic animals.
|
|
Nuclear Transfer Techniques
|
|
Cloning, Organism -- methods
|
|
Cell Differentiation -- genetics
|
|
Cell Nucleus -- physiology
|
|
Gene Transfer Techniques
|
|
Genetic Therapy
|
|
Animals, Genetically Modified
|
|
MEDICAL -- Research.
|
|
Cell nuclei -- Transplantation
|
|
Gene therapy
|
|
Transgenic animals
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Verma, Paul J.
|
|
Trounson, Alan
|
| ISBN |
1588292800 |
|
1597451541 |
|
9781597451543 |
|
9781588292803 |
|
1280832444 |
|
9781280832444 |
|
9786610832446 |
|
6610832447 |
|
