Title Synthetic mRNA : production, introduction into cells and physiological consequences / edited by Robert E. Rhoads

Published New York : Humana Press : Springer, ©2016

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Description 1 online resource (xii, 320 pages) : illustrations (some color) Series Methods in molecular biology ; 1428 Methods in molecular biology (Clifton, N.J.) ; v. 1428 Contents 880-01 Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences -- Synthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments -- Enzymatic Modification of 5'-Capped RNA and Subsequent Labeling by Click Chemistry -- Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG -- Intronless b-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements -- Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA -- Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells -- Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System -- GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use -- Large Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems -- FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines -- Transfecting Human Monocytes with RNA -- In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated byPiggyBac Transposition -- Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage -- Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy -- Immune Monitoring using mRNA-Transfected Dendritic Cells -- Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2 -- mRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70) -- Redirecting T Cell Specificity Using T Cell Receptor Messenger RNA Electroporation -- Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA -- Genetic Modification of Human Pancreatic Progenitor Cells through Modified mRNA 880-01/(S Synthetic mRNA : production, introduction into cells, and physiological consequences / Robert E. Rhoads -- Synthetic capped mRNAs for cap-specific photo-cross-linking experiments / Joanna Kowalska, Franck Martin, and Jacek Jemielity -- Enzymatic modification of 50-capped RNA and subsequent labeling by click chemistry / Josephin M. Holstein, Daniela Stummer, and Andrea Rentmeister -- Preparation of functional, fluorescently labeled mRNA capped with anthraniloyl-m7GpppG / Artem V. Domashevski [and others] -- Intronless β-Globin reporter : a tool for studying nuclear RNA stability elements / Jessica A. Brown and Joan A. Steitz -- Synthetic mRNA with superior properties that mimics the intracellular fates of natural histone mRNA / Wei Su [and others] -- Engineering WT1-encoding mRNA to increase translational efficiency in dendritic cells / Daphne Benteyn [and others] -- Electroporation of alphavirus RNA translational reporters into fibroblastic and myeloid cells as a tool to study the innate immune system / Christina L. Gardner [and others] -- GMP-grade mRNA electroporation of dendritic cells for clinical use / Judith Derdelinckx, Zwi N. Berneman, and Nathalie Cools -- Large-scale mRNA transfection of dendritic cells by electroporation in continuous flow systems / David Selmeczi -- FLT3 ligand as a molecular adjuvant for naked RNA vaccines / Sebastian Kreiter [and others] -- Transfecting human monocytes with RNA / Jens Dannull and Smita K. Nair -- In vitro synthesis, delivery, and bioavailability of exogenous mRNA in gene transfer mediated by PiggyBac transposition / Solenne Bire, Nicole Ishac, and Florence Rouleux-Bonnin -- Transfection of human keratinocytes with nucleoside-modified mRNA encoding CPD-photolyase to repair DNA damage / Gabor Boros [and others] -- Delivery of synthetic mRNA encoding FOXP3 antigen into dendritic cells for inflammatory breast cancer immunotherapy / Gayathri R. Devi and Sritama Nath -- Immune monitoring using mRNA-transfected dendritic cells / Troels Holz Borch, Inge Marie Svane, and Ozcan Met -- Transfection of tumor-infiltrating T cells with mRNA Encoding CXCR2 / Manja Idorn [and others] -- mRNA electroporation of dendritic cells with WT1, survivin, and TriMix (a mixture of caTLR4, CD40L, and CD70) / An Coosemans [and others] -- Redirecting T cell specificity using T cell receptor messenger RNA electroporation / Sarene Koh, Noriko Shimasaki, and Antonio Bertoletti -- Measuring hematocrit in mice injected with in vitro-transcribed erythropoietin mRNA / Azita Josefine Mahiny and Katalin Kariko -- Genetic modification of human pancreatic progenitor cells through modified mRNA / Song Lu [and others] Summary This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology Bibliography Includes bibliographical references and index Notes Online resource; title from PDF of title page (Springer Protocols, viewed May 31, 2016) Subject Messenger RNA -- Laboratory manuals RNA, Messenger -- chemical synthesis Cell Engineering -- methods Cell Differentiation -- genetics Medical genetics. Medical -- Genetics. Messenger RNA Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Rhoads, Robert E., 1944- ISBN 9781493936250 1493936255 1493936239 9781493936236

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