| Description |
1 online resource (213 pages) |
| Contents |
Intro -- Title Page -- Copyright Page -- Contents -- Preface -- Acknowledgments -- Nomenclature -- Chapter 1 Introduction -- Mammalian Cells: Modern Workhorses -- Products from Cells -- Cells as Therapeutic Agents -- Biomarkers for Health or Disease -- In Vitro Models -- Bridging the Gap -- The Preservation Toolkit -- Hypothermic Storage -- Cryopreservation -- Vitrification -- Dry State Storage -- Fit-for-Purpose -- One Size Does Not Fit All -- The Process is the Product -- Reproducibility -- Safety -- Dispelling the Myth of the Cold Black Box -- References -- Chapter 2 Pre-freeze Processing and Characterization -- Pre-freeze Processing -- Digestion of Cells from Intact Tissue -- Hypothermic Storage -- Selection of Subpopulations -- Activation or Stimulation -- Genetic Modification -- Culture -- Pre-freeze Process Monitoring -- Pre-freeze Characterization -- Identity -- Genetic Stability -- Enumeration -- Purity -- Adventitious Agents -- Microbial Testing of Cell Therapy Products -- Special Considerations for the Characterization of Cell Therapies -- Annotation of Pre-freeze Processing -- Scientific Principles -- Putting Principles into Action -- References -- Chapter 3 Formulation and Introduction of Cryopreservation Solutions -- Importance of Cryoprotective Agents -- Mechanisms of Cryoprotection -- Formulating a Cryopreservation Solution -- Formulation of a Vitrification Solution -- Characterization and Quality Control for Cryoprotective Solutions -- Toxicity of CPAs -- Osmotic Toxicity -- Biochemical Toxicity -- Developing a Protocol for Introducing CPA Solutions -- The Basic Experiment -- Introduction of Vitrification Solutions -- Cell Concentration -- Removal of CPA Solution -- Safety Considerations for Cryopreservation Solutions -- Cryopreservation Containers -- Overwraps -- Labeling -- Sample Annotation -- Scientific Principles |
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Putting Principles into Practice -- References -- Chapter 4 Freezing Protocols -- Importance of Cooling Rate -- Controlled-rate Freezing -- Controlled Cooling-rate Protocols -- Segment 1: Initial Hold Period -- Segment 2: Cooling -- Uncontrolled Nucleation -- Manual Nucleation -- Automatic Nucleation -- Verifying Segment 2 (Including S2a) -- "Delayed" Latent Heat -- Segment 3 -- Verifying Segment 3 -- Other Types of Controlled-rate Protocols -- Passive Freezing -- Transfer to Storage -- Vitrification -- Independent Temperature Measurement -- Scientific Principles -- Putting Principles into Practice -- References -- Chapter 5 Storage and Shipping of Frozen Cells -- Scientific Basis for Selection of a Storage Temperature -- Additional Considerations for Vitrified Samples -- Standards, Guidelines, and Best Practices -- Facilities -- Storage Equipment and Environment -- Mapping Storage Devices and Setting Alarm Limits -- Monitoring Systems -- Safety -- Inventory Management System -- Stability in Storage -- Temperature Fluctuations -- Influence of Background Ionizing Radiation on Stability in Storage -- Shelf-Life of Samples in Storage -- Fit-for-Purpose Storage Practices -- Risk Mitigation in Long-Term Storage -- Shipping or Transport of Cells -- General Shipping Considerations -- Liquid Nitrogen Dry Shippers -- Temperature Mapping of a Shipper -- Packaging of Samples Being Shipped -- Monitoring of Shipments -- Responsibilities -- Sample Annotation -- Scientific Principles -- Putting Principles into Practice -- References -- Chapter 6 Thawing and Post-Thaw Processing -- Thawing Equipment -- Transporting Samples Prior to Thawing -- Estimating Your Thawing Rate -- Thawing and Infusion of Cell Therapy Products -- Safety Considerations for Thawing -- Post-Thaw Processing -- Post-Thaw Washing -- Dilution -- Infusion of Cells Immediately Post-Thaw |
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Removal of Vitrification Solutions -- Wash Solutions -- Scientific Principles -- Putting Principles into Practice -- References -- Chapter 7 Post-Thaw Assessment -- Common Measures Used in Post‐Thaw Assessment -- Physical Integrity -- Metabolic Activity -- Mechanical Activity -- Mitotic Activity -- Differentiation Potential -- Transplantation Potential -- Strategies to Improve the Accuracy and Reproducibility of Post-Thaw Assessment -- Eliminate Measurement Bias -- Compensating for Post-Thaw Apoptosis -- Post-Thaw Assessment Using a Single Measure -- Optical Methods of Post‐Thaw Assessment -- Release Criteria -- Scientific Principles -- Putting Principles into Practice -- References -- Chapter 8 Algorithm-Driven Protocol Optimization -- Small Cell Number/High Throughput Approach -- Validating Operation of the Algorithm -- Flexibility -- Practical Notes -- Modeling in Cryobiology -- References -- Introduction -- ProtocolContributors -- Cryopreservation of Endothelial Cells in Suspension -- Principle -- Equipmentand Supplies -- Equipment -- Supplies -- Safety -- Procedure -- Cell Preparation -- Preparation of Cryoprotectant Solution -- Using Powdered HES -- Using Pentastarch Solution -- Cryoprotectant Addition -- Freezing -- Controlled-rate Freezing with a Methanol Bath -- Alternative Freezing Procedure -- Thawing -- Expected Results -- References -- Cryopreservation of Peripheral Blood Mononuclear Cells from Whole Blood -- Principle -- Protocol 1: Isolation of PBMCS Directly over Ficoll-Hypaque -- Equipment -- Materials -- Reagents -- Procedure -- Protocol 2: Isolation of PBMCS Using SepMates -- Equipment -- Materials -- Reagents -- Procedure -- Materials -- Appendix A Human Serum AB Freezing Media -- Equipment -- Reagents -- Procedure -- Cryopreservation of Human Adipose Stem Cells -- Principle -- Equipmentand Supplies -- Reagentsand Media |
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Procedure -- Isolation of Human ASCs from Lipoaspirate -- Magnetic Cell Sorting (Optional) -- Cryopreservation -- Controlled-rate Freezing of Human ASCs -- Thawing Human ASCs -- Notes -- Reference -- Cryopreservation of Red Blood Cells -- MethodI: High Glycerol/Slow Cooling Technique (Meryman and Hornblower 1972) -- Preparation of the RBC Concentrate -- Addition of the Cryoprotective Solution -- Cooling -- Rewarming -- Removal of the Cryoprotectant and Debris -- MethodII: A Low Glycerol/Rapid Cooling Technique (Rowe, Eyster, and Kellner 1968) -- MethodIII: Hydroxyethylstarch/Rapid Cooling Technique (Sputtek 2007) -- References -- Cryopreservation of Oocytes by Slow Freezing -- Principle -- SpecimenRequirements -- Equipmentand Supplies Needed -- Equipment -- Supplies -- Procedure -- Safety -- Calculations -- Reporting Results -- ProcedureNotes -- Limitationsof Procedure -- Oocyte Vitrification and Warming -- Principle -- Equipmentand Supplies -- Equipment -- Supplies -- Procedure -- QualityControl -- Safety -- Transportation of Hematopoietic Progenitor Cells and Other Cellular Products -- Principle/Rationale -- Specimen -- Equipment/Reagents -- QualityControl -- Procedure -- AdditionalInformation -- FurtherReading -- Cryopreservation of Hematopoietic Progenitor Cells -- Principle/Rationale -- Specimen -- Equipment/Reagents -- QualityControl -- Procedure -- Appendix A Alternate Cryopreservation HarnessSet-2 or 4 Bags -- FurtherReading -- Thawing of Hematopoietic Progenitor Cells -- Principle/Rationale -- Equipment/Reagents -- QualityControl -- Procedure -- FurtherReading -- Processing and Cryopreservation of T-Cells -- Principle/Rationale -- Protocol/Processing Schema: N/A -- Specimens -- Equipment/Reagents -- QualityControl -- Procedure -- FurtherReading -- Thawing and Reinfusion of Cryopreserved T-Cells -- Principle/Rationale |
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Protocol/Processing Schema -- Specimen -- Equipment/Reagents -- QualityControl -- Procedure -- Further Reading -- Index -- EULA |
| Notes |
Print version record |
| Subject |
Cells-Cryopreservation-Handbooks, manuals, etc
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Cells-Preservation-Handbooks, manuals, etc
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| Form |
Electronic book
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| ISBN |
9781118989852 |
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1118989856 |
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