| Description |
1 online resource (xii, 304 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; v. 1580 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1580. 1064-3745
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| Contents |
Improved denaturation of small RNA duplexes and its application for northern blotting / C. Jake Harris, David C. Baulcombe, and Attila Molnar -- 2 High-throughput RT-qPCR for the analysis of circulating MicroRNAs / Geok Wee Tan and Lu Ping Tan -- Genome-wide comparison of next-generation sequencing and qPCR platforms for microRNA profiling in serum / Thorarinn Blondal, Maurizia Rossana Brunetto, Daniela Cavallone, Martin Mikkelsen, Michael Thorsen, Yuan Mang, Hazel Pinheiro, Ferruccio Bonino, and Peter Mouritzen -- Small RNA profiling by next-generation sequencing using high-definition adapters / Martina Billmeier and Ping Xu -- Surface acoustic wave lysis and ion-exchange membrane quantification of exosomal MicroRNA / Katherine E. Richards, David B. Go, and Reginald Hill -- Droplet microfluidic device fabrication and use for isothermal amplification and detection of MicroRNA / Maria Chiara Giuffrida, Roberta D'Agata, and Giuseppe Spoto -- Interrogation of functional miRNA-target interactions by CRISPR/Cas9 genome engineering / Yale S. Michaels, Qianxin Wu, and Tudor A. Fulga -- Cell-free urinary MicroRNAs expression in small-scale experiments / Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger, Lubos Minar, Ales Horinek, and Milada Kohoutova -- Peptide-based isolation of argonaute protein complexes using Ago-APP/ Judith Hauptmann and Gunter Meister -- Predicting functional MicroRNA-mRNA interactions / Zixing Wang and Yin Liu -- Computational and experimental identification of tissue-specific microRNA targets / Raheleh Amirkhah, Hojjat Naderi Meshkin, Ali Farazmand, John E.J. Rasko, and Ulf Schmitz -- sRNAtoolboxVM : small RNA analysis in a virtual machine / Cristina Gómez-Martín, Ricardo Lebrón, Antonio Rueda, José L. Oliver, and Michael Hackenberg -- Assessment of the next generation of animal miRNA target prediction algorithms / Thomas Bradley and Simon Moxon -- UEA small RNA workbench : a suite of computational tools for small RNA analysis / Irina Mohorianu, Matthew Benedict Stocks, Christopher Steven Applegate, Leighton Folkes, and Vincent Moulton -- Prediction of miRNA-mRNA interactions using miRGate / Eduardo Andrés-León, Gonzalo Gómez-López, and David G. Pisano -- Detection of microRNAs using chip-based QuantStudio 3D digital PCR / Cristina Borzi, Linda Calzolari, Davide Conte, Gabriella Sozzi, and Orazio Fortunato -- MiRNA quantitation with microelectrode sensors enabled by enzymeless electrochemical signal amplification / Tanyu Wang, Gangli Wang, Didier Merlin, and Emilie Viennois -- Robust protocol to quantify circulating cancer biomarker microRNAs / Emma Bell, Hannah L. Watson, Shivani Bailey, Matthew J. Murray, and Nicholas Coleman -- MicroRNAs, regulatory networks, and comorbidities : decoding complex systems / Francesco Russo, Kirstine Belling, Anders Boeck Jensen, Flavia Scoyni, Søren Brunak, and Marco Pellegrini -- Label-free direct detection of MiRNAs with poly-silicon nanowire biosensors / Jing He, Jianjun Zhu, Bin Jiang, and Yulan Zhao -- Erratum to : Cell-free urinary MicroRNAs expression in small-scale experiments / Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger, Lubos Minar, Ales Horinek, and Milada Kohoutova |
| Summary |
This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed April 25, 2017) |
| Subject |
MicroRNA -- Laboratory manuals
|
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MicroRNAs -- chemistry
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MicroRNAs -- genetics
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Computational Biology -- methods
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Sequence Analysis, RNA -- methods
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MicroRNA
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Dalmay, Tamas, editor
|
| ISBN |
9781493968664 |
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1493968661 |
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1493968645 |
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9781493968640 |
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