| Description |
1 online resource (x, 327 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 11 |
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Methods in molecular biology (Clifton, N.J.) ; v. 11.
|
| Contents |
Thiophilic adsorption chromatography / T. William Hutchens -- Immobilized metal ion affinity chromatography / Tai-Tung Yip and T. William Hutchens -- Histidine ligand affinity chromatography / Mookambeswaran A. Vijayalakshmi -- Boronic acid matrices for the affinity purification of glycoproteins and enzymes / James H. Hageman and Glenn D. Kuehn -- Calcium-dependent hydrophobic interaction chromatography / Nicholas H. Battey and Michael A. Venis -- Lectin affinity chromatography / Iris West and Owen Goldring -- Dye-ligand affinity chromatography / Steven J. Burton -- High-performance affinity chromatography for protein separation and purification / Yannis D. Clonis -- Immunoaffinity chromatography / George W. Jack -- Selection of antibodies for immunoaffinity chromatography / Duncan S. Pepper -- Some alternative coupling chemistries for affinity chromatography / Duncan S. Pepper -- Exploiting weak affinities / Sten Ohlson -- Biospecific affinity elution / Robert Kerry Scopes -- Size-exclusion high-performance liquid chromatography of proteins / David A. Harris -- Chromatofocusing / Chee Ming Li and T. William Hutchens -- Ion-exchange chromatography of proteins / Andrew C. Kenney -- Displacement chromatography of proteins / Steven M. Cramer -- Purification of DNA binding proteins by affinity and ion exchange chromatography / David Hornby, Kevin Ford and Paul Shore -- Making bacterial extracts suitable for chromatography / Roger F. Sherwood -- Determination of purity and yield / Sudesh B. Mohan |
| Summary |
"Kenney and Foweh's exceptionally useful new book, Practical Protein Chromatography, brings all the forefront methods of protein separation and purification directly to your laboratory benchtop. The book treats not only powerful analytical separations, but also the small- and large-scale preparative techniques that are currently applicable throughout biological and biomedical research." "Chapters, written by experts in each of the techniques covered, treat a variety of methods usable in virtually every laboratory with a chromatographic system. Topics include immunoaffinity chromatography and selection of antibodies, lectin affinity and dye-ligand chromatography, exploiting weak affinities, alternative coupling chemistries, biospecific affinity elution, size-exclusion HPLC of proteins, chromatofocusing, determination of purity and yield, and specialized chromatographic techniques." "The established tradition of Methods in Molecular Biology is to present techniques that guarantee optimum results. Everyone doing protein chromatography today--at any level of proficiency--will find Practical Protein Chromatography an indispensable, hands-on guide to the effective use of these methodologies."--Jacket |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Proteins -- Analysis.
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Affinity chromatography.
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Proteins -- analysis
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Chromatography, Affinity
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Affinity chromatography
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Proteins -- Analysis
|
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Eiwitten.
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Chromatografie.
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Wetenschappelijke technieken.
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Proteinas.
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Cromatografia.
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Biologia molecular e macromolecular.
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| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Kenney, Andrew
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|
Fowell, Susan
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| ISBN |
9781592594986 |
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1592594980 |
|
