| Description |
1 online resource (xv, 528 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 119 |
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Methods in molecular biology (Clifton, N.J.) ; v. 119
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| Contents |
Expression and purification of recombinant histones and nucleosome reconstitution / K. Luger, T.J. Rechsteiner and T.J. Richmond -- Preparation and analysis of positioned nucleosomes / V.M. Studitsky -- Site-directed chemical probing of histone-DNA interactions / D.R. Chafin, K.M. Lee and J.J. Hayes -- Base-pair resolution mapping of nucleosomes in vitro / A. Flaus and T.J. Richmond -- Equilibrium and dynamic nucleosome stability / J. Widom -- Nucleosome structure and dynamics. The DNA minicircle approach / A. Prunell, M. Alilat and F. De Lucia -- Analysis of linker histone binding to mono- and dinucleosomes / S. Chandler and A.P. Wolffe -- Quantitative analysis of chromatin higher-order organization using agarose gel electrophoresis / J.C. Hansen, T.M. Fletcher and J.I. Kreider -- Analytical ultracentrifugation of chromatin / J.C. Hansen and C.L. Turgeon -- Analysis of chromatin by scanning force microscopy / S.H. Leuba and C. Bustamante -- In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension / R.E. Wellinger [and others] -- Preparation of chromatin assembly extracts from Xenopus oocytes / D.J. Tremethick -- Preparation of chromatin assembly extracts from preblastoderm drosophila embryos / E. Bonte and P.B. Becker -- A solid-phase approach for the analysis of reconstituted chromatin / R. Sandaltzopoulos and P.B. Becker -- Reconstitution and analysis of hyperacetylated chromatin / W.A. Krajewski and P.B. Becker -- Assembly of mitotic chromosomes in Xenopus egg extract / A.E. de la Barre, M. Robert-Nicoud and S. Dimitrov -- Nucleotide excision repair coupled to chromatin assembly / P.H. Gaillard, D. Roche and G. Almouzni -- Photolyase. A molecular tool to characterize chromatin structure in yeast / M. Livingstone-Zatchej, B. Suter and F. Thoma -- Transcriptional and structural analyses of isolated SV40 chromatin / U. Hansen -- In vitro replication of chromatin templates / C. Gruss -- Analysis of HMG-14/-17-containing chromatin / Y.V. Postnikov and M. Bustin -- Identification and analysis of native nucleosomal histone acetyltransferase complexes / P.A. Grant, S.L. Berger and J.L. Workman -- Analysis of nucleosome disruption by ATP-driven chromatin remodeling complexes / T. Owen-Hughes [and others] -- Nucleosome remodeling factor NURF and in vitro transcription of chromatin / G. Mizuguchi and C. Wu -- An SDS-PAGE-based enzyme activity assay for the detection and identification of histone acetyltransferases / J.E. Brownell, C.A. Mizzen and C.D. Allis -- Analysis of DNaseI hypersensitive sites in chromatin by cleavage in permeabilized cells / R. Aasland and A.F. Stewart -- Mapping of nucleosome positions in yeast / M. Livingstone-Zatchej and F. Thoma -- Analysis of DNA topology in yeast chromatin / R.H. Morse -- DNA methyltransferases as probes for chromatin structure in yeast / M.P. Kladde, M. Xu and R.T. Simpson -- Restriction nucleases as probes for chromatin structure / P.D. Gregory, S. Barbaric and W. Horz -- Genomic footprinting using nucleases / L. Cappabianca [and others] -- In situ analysis of chromatin proteins during development and cell differentiation using flow cytometry / D. Grunwald [and others] -- Mapping DNA target sites of chromatin proteins in vivo by formaldehyde crosslinking / H. Strutt and R. Paro -- Mapping DNA interaction sites of chromosomal proteins. Crosslinking studies in yeast / A. Hecht, S. Strahl-Bolsinger and M. Grunstein -- UV laser footprinting and protein-DNA crosslinking. Application to chromatin / D. Angelov, S. Khochbin and S. Dimitrov -- An in vivo UV crosslinking assay that detects DNA binding by sequence-specific transcription factors / A. Carr and M.D. Biggin |
| Summary |
The state-of-the-art methods collected here are designed to analyze the relationship between chromatin structure and function, and to elucidate the molecular mechanisms that control such vital cellular functions as transcription, replication, recombination, and DNA repair. These proven methods cover a wide range of topics, from powerful cell-free systems for the reconstitution of chromatin heterogeneity in vitro, to both classical and cutting-edge techniques for in vivo analysis of protein-DNA interactions. Each method includes detailed step-by-step instructions to ensure successful replication along with helpful notes about how to avoid pitfalls. All authors are leading scientists, well known for their methodological expertise in the chromatin research |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Chromatin -- Laboratory manuals
|
|
Chromatin -- metabolism
|
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Chromatin -- ultrastructure
|
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SCIENCE -- Life Sciences -- Molecular Biology.
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Chromatin
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Becker, Peter B.
|
| ISBN |
0896036650 |
|
9780896036659 |
|
9781592596812 |
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1592596819 |
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