Title Corneal regenerative medicine : methods and protocols / edited by Bernice Wright, Che J. Connon

Published New York : Humana Press, ©2013

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Description 1 online resource (xii, 230 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 1014 Methods in molecular biology (Clifton, N.J.) ; v. 1014. 1064-3745 Contents Limbal epithelial cell therapy : past, present, and future / Tor Paaske Utheim -- Artificial cornea / Naresh Polisetti, Mohammad Mirazul Islam, and May Griffith -- Clonal analysis of limbal epithelial stem cell populations / Ursula Schlötzer-Schrehardt -- Use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation / Ashley M. Crane and Sanjoy K. Bhattacharya -- Enrichment of human corneal epithelial stem/progenitor cells by magnetic bead sorting using SSEA4 as a negative marker / Martin N. Nakatsu and Sophie X. Deng -- Limbal epithelial stem cell identification using immunoblotting analysis / Bernice Wright and Che J. Connon -- Culture of limbal epithelial cells / Tor Paaske Utheim, Torstein Lyberg, and Sten Ræder -- Culture of limbal stromal cells and corneal endothelial cells / Naresh Polisetti and Nancy C. Joyce -- Formation of a tissue-engineered cornea using plastically compressed collagen scaffolds and limbal stem cells / Shengli Mi and Che J. Connon -- Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry / Mohammad Mirazul Islam, May Griffith, and Kimberley Merrett -- Fabrication of a corneal-limbal tissue substitute using silk fibroin / Laura J. Bray [and others] -- Cultivation of limbal epithelial cells on electrospun poly(lactide-co-glycolide) scaffolds for delivery to the cornea / Pallavi Deshpande [and others] -- Growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds / Vladimir Holan, Eliska Javorkova, and Peter Trosan -- Encapsulation and culture of mammalian cells including corneal cells in alginate hydrogels / Nicola C. Hunt and Liam M. Grover -- In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans / Neil Lagali, May Griffith, and Per Fagerholm Summary Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings Analysis Medicine Ophthalmology Biomedicine Molecular Medicine geneeskunde biomedische wetenschappen oogheelkunde Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Subject Cornea -- Regeneration -- Laboratory manuals Regenerative medicine -- Laboratory manuals Anterior segment (Eye) Epithelium. Surgery, Plastic. Transplantation of organs, tissues, etc. Cultures (Biology) Medical laboratory technology. Eye. Medical personnel. Surgery, Operative. Anatomy. Sense organs. Tissue engineering. Cornea. Guided tissue regeneration. Regenerative medicine. Cornea -- Transplantation. Anterior Eye Segment Epithelium Plastic Surgery Procedures Refractive Surgical Procedures Tissue Transplantation Publication Formats Medicine Culture Techniques Tissues Clinical Laboratory Techniques Eye Health Occupations Publication Characteristics Surgical Procedures, Operative Ophthalmologic Surgical Procedures Transplantation Investigative Techniques Disciplines and Occupations Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Sense Organs Tissue Engineering Epithelium, Corneal Cornea Laboratory Manuals Guided Tissue Regeneration Regenerative Medicine Corneal Transplantation anatomy. Transplantation of organs, tissues, etc. Tissue engineering Surgery, Plastic Surgery, Operative Sense organs Medical personnel Medical laboratory technology Guided tissue regeneration Eye Epithelium Cultures (Biology) Cornea -- Transplantation Cornea Anterior segment (Eye) Anatomy Regenerative medicine Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Wright, Bernice Connon, Che J ISBN 9781627034326 1627034323 1627034315 9781627034319

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