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Title Drosophila cytogenetics protocols / edited by Daryl S. Henderson
Published Totowa, N.J. : Humana Press, ©2004


Description 1 online resource (xiii, 468 pages) : illustrations
Series Methods in molecular biology ; v. 247
Methods in molecular biology (Clifton, N.J.) ; v. 247
Contents The chromosomes of Drosophila melanogaster / D.S. Henderson -- Spermatogenesis: analysis of meiosis and morphogenesis / H. White-Cooper -- Time-lapse imaging of male meiosis by phase-contrast and fluorescence microscopy / E. Rebollo and C. Gonzalez -- Immunocytological analysis of oogenesis / E. Mathe -- Cytological analysis of oogenesis / S. Nokkala and C. Nokkala -- Polytene chromosomes from ovarian nurse cells of Drosophila melanogaster otu mutants / D.E. Koryakov [and others] -- Feulgen-DNA cytophotometry for estimating C values / E.M. Rasch -- Fluorescent BrdU labeling and nuclear flow sorting of the Drosophila ovary / B.R. Calvi and M.A. Lilly -- Studying nuclear organization in embryos using antibody tools / K.M. Johansen and J. Johansen -- Whole-mount fluorescence in situ hybridization to chromosomes of embryos / D.S. Henderson -- Orcein staining and the identification of polytene chromosomes / J. Tonzetich -- Salivary chromosome analysis of aberrations / A.T. Carpenter -- In situ hybridization to polytene chromosomes / R.D. Saunders -- Combined immunostaining and FISH analysis of polytene chromosomes / S. Lavrov, J. Dejardin and G. Cavalli -- Electron microscopy of polytene chromosomes / V.F. Semeshin [and others] -- Analysis of mitosis in squash preparations of larval brains: orcein, Giemsa, Hoechst 33258, DAPI, quinacrine, and N-banding / L. Fanti and S. Pimpinelli -- Analyzing chromosome function by high frequency formation of dicentric chromosomes in vivo / K. Ahmad and K.G. Golic -- Analysis of chromosomes of the larval CNS by FISH and BrdU labeling / A.K. Csink -- Immunostaining of squash preparations of chromosomes of larval brains / L. Fanti and S. Pimpinelli -- Visualizing mitosis in whole-mount larval brains / D.S. Henderson -- Immunostaining of whole-mount imaginal discs / S. Phalle Bde -- Wing somatic mutation and recombination test / H.H. de Andrade, M.L. Reguly and M. Lehmann -- Analysis of histoblasts / M.M. Madhavan and K. Madhavan -- Visualizing apoptosis / K. McCall [and others] -- RNAi in cultured Drosophila cells / L.R. Kao and T.L. Megraw
Summary Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators
Bibliography Includes bibliographical references and index
Notes Print version record
In Springer Protocols
Subject Drosophila melanogaster -- Cytogenetics -- Laboratory manuals
Drosophila melanogaster -- cytology
Drosophila melanogaster -- genetics
Cell Division -- genetics
Cytogenetic Analysis -- methods
NATURE -- Animals -- Insects & Spiders.
SCIENCE -- Life Sciences -- Zoology -- Entomology.
Drosophila melanogaster -- Cytogenetics
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Henderson, Daryl S
LC no. 2003010918
ISBN 9781592596652