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Title Reviews and protocols in DT40 research / edited by Jean-Marie Buerstedde and Shunichi Takeda
Published New York : Springer, 2006


Description 1 online resource (xii, 477 pages) : illustrations (some color)
Series Subcellular biochemistry, 0306-0225 ; v. 40
Sub-cellular biochemistry ; v. 40.
Contents Ch. 1. DT40 gene disruptions: a how-to for the design and the construction of targeting vectors / Hiroshi Arakawa and Jean-Marie Buerstedde -- Ch. 2. Immunoglobulin gene conversion or hypermutation: That's the question / Jean-Marie Buerstedde and Hiroshi Arakawa -- Ch. 3. Genome resources for the DT40 community / Randolph B Caldwell and Andrzej M. Kierzek -- Ch. 4. Chromosome engineering in DT40 cells and mammalian centromere function / William R.A. Brown [and others] -- Ch. 5. Function of recQ family helicase in genome stability / Masayuki Seki, Shusuke Tada and Takemi Enomoto -- Ch. 6. Genetic analysis of apoptotic execution / Sandrine Ruchaud [and others] -- Ch. 7. The DT40 system as a tool for analyzing kinetochore assembly / Masahiro Okada, Tesuya Hori and Tatsuo Fukagawa -- Ch. 8. Analysing the DNA damage and replication checkpoints in DT40 cells / Michael D Rainey, George Zachos, and David AF Gillespie -- Ch. 9. Using DT40 to study clathrin function / Frank R. Wettey and Antony P. Jackson -- Ch. 10. Genetic analysis OF B cell signaling / Hisaaki Shinohara and Tomohiro Kurosaki
Summary Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Vertebrates -- Cytogenetics -- Handbooks, manuals, etc
Genetic regulation -- Methodology -- Handbooks, manuals, etc
Genetics -- Technique.
Vertebrae -- Cytogenetics -- Handbooks, manuals, etc
Vertebrates -- genetics
Genetic Techniques
SCIENCE -- Life Sciences -- Zoology -- General.
Vertebrates -- Genetics.
Genetic Techniques.
Vertébrés -- Cytogénétique.
Régulation génétique -- Méthodologie.
Vertebrates -- Cytogenetics.
Genetic regulation -- Methodology.
Sciences de la vie.
Genetics -- Technique
Genre/Form handbooks.
Handbooks and manuals
Handbooks and manuals.
Guides et manuels.
Form Electronic book
Author Buerstedde, Jean-Marie.
Takeda, Shunʼichi.
ISBN 9781402048968