| Description |
1 online resource (xiv, 550 pages) : illustrations |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; 612 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 612
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| Contents |
Purification of human NK cell developmental intermediates from lymph nodes and tonsils / Aharon G. Freud and Michael A. Caligiuri -- In vitro development of human killer-immunoglobulin receptor-positive NK cells / Frank Cichocki and Jeffrey S. Miller -- Subset analysis of human and mouse mature NK cells / Yoshihiro Hayakawa, Daniel M. Andrews, and Mark J. Smyth -- Assessing licensing of NK cells / A. Helena Jonsson and Wayne M. Yokoyama -- Use of stem cell radiation chimeras to analyze how domains of specific proteins impact on murine NK cell development in vivo / Rebecca H. Lian and Vinay Kumar -- Use of transfected Drosophila S2 cells to study NK cell activation / Michael E. March, Catharina C. Gross, and Eric O. Long -- Natural killer cell conjugate assay using two-color flow cytometry / Deborah N. Burshtyn and Chelsea Davidson -- Studying NC cell/dendritic cell interactions / Mathias Lucas, Cedric Vonarbourg, Peter Aichele, and Andreas Diefenbach -- Analysis of the NK cell immunological synapse / Keri B. Sanborn, Gregory D. Rak, Ashley N. Mentlik, Pinaki P. Banerjee, and Jordan S. Orange -- Measuring intracellular calcium signaling in murine NK cells by flow cytometry / Alexander W. MacFarlane IV, James F. Oesterling, and Kerry S. Campbell -- Intracellular staining for analysis of the expression and phosphorylation of signal transducers and activators of transcription (STATs) in NK cells / Takuya Miyagi, Seung-Hwan Lee, and Christine A. Biron -- A model system for studying NK cell receptor signaling / Lukasz K. Chlewicki and Vinay Kumar -- Expression of cDNAs in human natural killer cell lines by retroviral transduction / S.M. Shahjahan Miah and Kerry S. Campbell -- Lentiviral gene transduction in human and mouse NK cell lines / Ram Savan, Tim Chan, and Howard A. Young -- Introduction of shRNAs into human NK-like cell lines with retrovirus / Amanda K. Purdy and Kerry S. Campbell -- Introduction of shRNAs iinto primary NK cells with lentivirus / Sam K.P. Kung -- Methods to identify and characterize different NK cell receptors and their ligands / Dikla Lankry, Roi Gazit, and Ofer Mandelboim -- Generating NK cell receptor-Fc chimera proteins from 293T cells and considerations of appropriate glycosylation / Alon Zilka, Michael Mendelson, Benyamin Rosental, Oren Hershkovitz, and Angel Porgador -- Identification of NK cell receptor ligands using a signaling reporter system / Yoshie-Matsubayashi Iizuka, Nikunj V. Somia, and Koho Iizuka -- Determining ligand specificity of Ly49 receptors / Kerry J. Lavender and Kevin P. Kane -- Probing the interactions of NK cell receptors with ligand expressed in trans and cis / Jonathan Back, Léonardo Scarpellino, and Werner Held -- A simple method to measure NK cell cytotoxicity in vivo / Aurore Saudemont, Shannon Burke, and Francesco Colucci -- Functional analysis of human NK cells by flow cytometry / Yenan T. Bryceson, Cyril Fauriat, João M. Nunes, Stephanie M. Wood, Niklas K. Björkström, Eric O. Long, and Hans-Gustaf Ljunggren -- Analysis of the KIR repertoire in human NK cells by flow cytometry / Niklas K. Björkström, Cyril Fauriat, Yenan T. Bryceson, Johan K. Sandberg, Hans-Gustaf Ljunggren, Kari-Johan Malmberg -- KIR genotyping by multiplex PCR-SSP / Smita Kulkarni, Maureen P. Martin, and Mary Carrington -- Identification and analysis of novel transcripts and promoters in the human killer cell immunoglobulin-like receptor (KIR) genes / Hongchuan Li, Paul W. Wright, and Stephen K. Anderson -- Use of inbred mouse strains to map recognition receptors of MCMV infected cells in the NK cell gene locus / Nassima Fodil-Cornu, Michal Pyzik, and Silvia M. Vidal -- Studying NK cell responses to ectromelia virus infections in mice / Min Fang and Luis Sigal -- Activation of human NK cells by malaria-infected red blood cells / Amir Horowitz and Eleanor M. Riley -- Natural killer cells in human pregnancy / Victoria Male, Anita Trundley, Lucy Gardner, Jacquie Northfield, Chiwen Chang, Richard Apps, and Ashley Moffett -- Analysis of uterine natural killer cells in mice / B. Anne Croy, Jianhong Zhang, Chandrakant Tayade, Francesco Colucci, Hakim Yadi, and Aureo T. Yamada -- Isolation of NK cells and NK-like cells from the intestinal lamina propria / Stephanie L. Sanos and Andreas Diefenbach |
| Summary |
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of Natural Killer Cell Protocols: Cellular and Molecular Methods brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells |
| Analysis |
natuurlijke afweercellen |
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natural killer cells |
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celbiologie |
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cellular biology |
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moleculaire biologie |
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molecular biology |
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laboratoriummethoden |
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laboratory methods |
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protocollen |
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protocols |
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immunologie |
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immunology |
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Biological Techniques |
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Biologische technieken |
| Notes |
Title from PDF title page (SpringerLink, viewed January 6, 2010) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Killer cells -- Laboratory manuals
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Killer cells.
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Cytotoxicity Tests, Immunologic -- methods
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Killer Cells, Natural -- immunology
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Killer cells
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Campbell, Kerry S.
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| ISBN |
9781607613626 |
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160761362X |
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