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Title CDNA libraries : methods and applications / edited by Chaofu Lu, John Browse and James G. Wallis
Published New York, NY : Humana, ©2011


Description 1 online resource (xi, 275 pages) : illustrations (some color)
Series Springer protocols
Methods in molecular biology, 1064-3745 ; 729
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 729
Contents Isolation of fungal infection structures from plant tissue by flow cytometry for cell-specific transcriptome analysis / H. Takahara, E. Endl and R. O'Connell -- Preparation of a high-quality cDNA library from a single-cell quantity of mRNA using chum-RNA / H. Nojima and T. Tougan -- Construction of a full-length cDNA library from castor endosperm for high-throughput functional screening / C. Lu, J.G. Wallis and J. Browse -- Full-length transcriptome analysis using a bias-free cDNA library prepared with the vector-capping method / S. Kato, M. Oshikawa and K. Ohtoko -- Construction of improved yeast two-hybrid libraries / R.H. Maier, C.J. Maier and K. Onder -- Normalization of full-length-enriched cDNA / E.A. Bogdanova [and others] -- Bioinformatic methods for finding differentially expressed genes in cDNA libraries, applied to the identification of tumour vascular targets / J.M. Herbert [and others] -- Enzymatic production of RNAi libraries from cDNAs and high-throughput selection of effective shRNA expression constructs / K. Sugao and K. Hirose -- Construction of small RNA CDNA libraries for high-throughput sequencing / C. Lu and V. Shedge -- Focusing mutations within random libraries to distinct areas: Protein domain library generation by overlap extension / A. Gratz and J. Jose -- Generation of families of construct variants using golden gate shuffling / C. Engler and S. Marillonnet -- Application of full-length cDNA resources to gain-of-function technology for characterization of plant gene function / Y. Kondou [and others] -- Construction of yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- Identification of protein/target molecule interactions using yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- SNP discovery by transcriptome pyrosequencing / W.B. Barbazuk and P.S. Schnable -- RNA-seq analysis of gene expression and alternative splicing by double-random priming strategy / M.T. Lovci [and others] -- Generation of a large catalog of unique transcripts for whole-genome expression analysis in nonmodel species / D. Bellin, A. Ferrarini and M. Delledonne
Summary The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years. In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries. The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology
Analysis genexpressie
gene expression
biomedische wetenschappen
humane genetica
human genetics
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Antisense DNA.
DNA, Complementary
Antisense DNA
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Lu, Chaofu.
Browse, John (John Anthony)
Wallis, James G.
ISBN 9781617790652