| Description |
1 online resource (x, 212 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 187 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 187
|
| Contents |
Agarose and polyacrylamide gel electrophoresis / Andrea M. Guilliatt -- Internal labeling of DNA probes / Ralph Rapley -- End-labeling of DNA probes / Adrian J. Harwood -- Southern blotting of agarose gels bu capillary transfer / Ralph Rapley -- Restriction fragment length polymorphism / Mohammad S. Enayat -- PCR: principles, procedures, and parameters / Nicola Louise Jones -- Allele-specific oligonucleotide PCR / Elaine K. Green -- Long-range PCR / Peter A. Davies -- Analysis of nucleotide sequence variations by solid-phase minisequencing / Anu Wartiovaara -- Cycle sequencing of PCR products / G.K. Surdhar -- Fluorescent in situ hybridization / Sara A. Dyer -- The protein truncation test / Carol A. Hardy -- Mutation detection in factor vii cDNA from lymphocytes of hemophilia A patients by solid phase fluorescent chemical cleavage of mismatch / Naushin H. Waseem -- Denaturing gradient gel electrophoresis / Yvonee Wallis -- Conformation-sensitive gel electrophoresis / Ian J. Williams -- SSCp/heteroduplex analysis -- Cleavase fragment length polymorphism analysis for genotyping and mutation detection / Laura Heisler -- Automated genotyping using the DNA MassArray technology / Christian Jurinke -- An introduction to bioinformatics / Henry Brzeski |
| Summary |
Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution. Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| In |
Springer Protocols |
| Subject |
Mutation (Biology) -- Laboratory manuals
|
|
Polymerase chain reaction -- Laboratory manuals
|
|
DNA Mutational Analysis -- methods
|
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Polymerase Chain Reaction -- methods
|
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SCIENCE -- Life Sciences -- Genetics & Genomics.
|
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Mutation (Biology)
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Polymerase chain reaction
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Theophilus, Bimal D. M
|
|
Rapley, Ralph
|
| ISBN |
1592592732 |
|
9781592592739 |
|
0896036170 |
|
9780896036178 |
|
0585403309 |
|
9780585403304 |
|
1280830581 |
|
9781280830587 |
|
9786610830589 |
|
6610830584 |
|
