Title PRINS and in situ PCR protocols / edited by John R. Gosden

Published Totowa, N.J. : Humana Press, ©1997

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Description 1 online resource (xiii, 165 pages) : illustrations Series Methods in molecular biology ; 71 Methods in molecular biology (Clifton, N.J.) ; 71 Contents Oligonucleotide PRINS DNA synthesis / John R. Gosden, Diane Lawson -- Chromosome-specific PRINS / Jean-Paul Charlieu, Franck Pellestor -- Bright-field microscopic detection of oligonucleotide PRINS-labeled DNA in chromosome preparations / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Analysis of sperm aneuploidy by PRINS / Franck Pellestor, Jean-Paul Charlieu -- PRINS DNA synthesis on frozen tissue sections / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Multiple sequential oligonucleotide primed in situ DNA syntheses (MULTI-PRINS) / John R. Gosden, Diane Lawson -- Oligonucleotide PRINS DNA synthesis on extended chromatin preparations / Yoshiro Shibasaki, John R. Gosden -- Combined immunocytochemistry and PRINS DNA synthesis for simultaneous detection of phenotypic and genomic parameters in cells / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Chromosomal PRINS DNA labeling combined with indirect immunocytochemistry / Arthur R. Mitchell -- Direct in situ single-copy (DISC)-PCR / Deryl L. Troyer, Hongyan Xie, Jie Hu -- In situ PCR to cells and to wax sections: an alternative to in situ hybridization for the detection of low copies of human papillomavirus and cytomegalovirus DNA / Christiane Mougin, Jean Marc Didier, Dominique Bettinger, Laurence Madoz, Sylviane Coumes-Marquet, Michel Lab -- Reverse transcriptase PCR in situ on cryopreserved tissue sections / Fred J. Hendler, Alice Shum-Siu, Briana W. Heniford, Mohammed El Ahmed, V.J. Patel -- Flow cytometry analysis of an in situ PCR for the detection of human immunodeficiency virus type-1 (HIV-1) proviral DNA / David E. Gibellini, Maria Carla Re, Giuliano Furlini, Michele Placa -- In situ PCR amplification of intracellular mRNA / Raymond H. Chen, Susan V. Fuggle -- Localized in situ amplification (LISA) : PCR amplification of nucleic acid sequences in tissue sections / Gregory J. Tsongalis -- In situ PCR : an overview / Aldan A. Long, Paul Komminoth Summary Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells Analysis oligonucleotiden oligonucleotides polymerase chain reaction chromosoomkaarten chromosome maps chromosomen chromosomes dna replication dna biologische technieken biological techniques protocollen protocols Molecular Biology (General) Cytogenetics Moleculaire biologie (algemeen) Cytogenetica Notes Source of title; title from title page Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject In situ hybridization -- Laboratory manuals Polymerase chain reaction -- Laboratory manuals DNA Primers -- chemistry Polymerase Chain Reaction -- methods Sequence Analysis -- methods Situ Hybridization -- methods Chromosome Mapping -- methods In situ hybridization Polymerase chain reaction Polymerase kettingreactie. Wetenschappelijke technieken. Genre/Form Handbooks, manuals, etc Handbooks and manuals Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Gosden, John R. ISBN 9781592595570 159259557X 0896033953 9780896033955

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