Title CDNA library protocols / edited by Ian G. Cowell and Caroline A. Austin

Published Totowa, N.J. : Humana Press, ©1997

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Description 1 online resource (x, 321 pages) : illustrations Series Methods in molecular biology ; v. 69 Methods in molecular biology (Clifton, N.J.) ; v. 69 Contents cDNA library construction from small amounts of RNA using paramagnetic beads and PCR / Kris N. Lambert, Valerie M. Williamson -- Increasing the average abundance of low-abundance cDNAs by Ordered subdivision of cDNA populations / David R. Sibson, Michael P. Starkey -- Isolation of messenger RNA from plant tissues / Alison Dunn -- cDNA library construction for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Clone excision methods for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Using rapid amplification of cDNA ends (RACE) to obtain full-length cDNAs / Yue Zhang, Michael A. Frohman -- Inverse PCR approach to cloning cDNA ends / Sheng-He Huang -- Cloning gene family members using the polymerase chain reaction with degenerate oligonucleotide primers / Gregory M. Preston -- Subtractive hybridization for the isolation of differentially expressed genes using magnetic beads / Hans-Christian Aasheim, Ton Logtenberg, Frank Larsen -- Preparation of competent cells for high-efficiency plasmid transformation of Escherichia coli / Yasuhiko Nakata, Xiaoren Tang, Kazunari K. Yokoyama -- The tetramethylammonium chloride (TMAC) method for screening cDNA libraries with highly degenerate oligonucleotide probes obtained by reverse translation of amino acid sequences / Bent Honoé, Peder Madsen -- Screening cDNA libraries by hybridization with double-stranded DNA probes and oligonucleotides / Caroline A. Austin -- Immunological screening of [lambda]-phage cDNA expression libraries / Helen C. Hurst -- Cloning sequence-specific DNA-binding factors from cDNA expression libraries using oligonucleotide binding site probes / Ian G. Cowell -- Protein interaction cloning by far-western screening of [lambda]-phage cDNA expression libraries / Shinichi Takayama, John C. Reed -- Yeast two-hybrid library screening / Ian G. Cowell -- Signal sequence trap : expression cloning method for secreted proteins and Type 1 membrane proteins / Kei Tashiro, Toru Nakano, Tasuku Honjo -- Isolation of genetic suppressor elements (GSEs) from random fragment cDNA libraries in retroviral vectors / Andrei V. Gudkov, Igor B. Roninson -- Expression and preparation of fusion proteins from recombinant [lambda]gt1.1 phages / Sheng-He Huang, Ambrose Jong -- Computer analysis of cloned sequences / Paul R. Caron -- The TIGR human cDNA database / Ewen F. Kirkness, Anthony R. Kerlavage -- Searching the dbEST database / Patricia Rodriguez-Tomé -- Reference cDNA library facilities available from European sources / Michael P. Starkey, Yagnesh Umrania, Christopher R. Mundy, Martin J. Bishop Summary This comprehensive collection of detailed protocols covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely used protocols with some very useful newer techniques. The protocols describe methods for cloning difficult-to-obtain ends of cDNAs, methods for analyzing cDNA sequence data, and methods for using the wealth of cDNA data emerging from the human genome project. Bearing in mind the importance of the library screening method to the determination of cloning strategy, the book offers a wide range of approaches to screening cDNA libraries Analysis complementair dna complementary dna dna libraries biologische technieken biological techniques protocollen protocols Molecular Biology (General) Moleculaire biologie (algemeen) Notes Source of title; title from title screen Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Gene libraries -- Laboratory manuals DNA -- Laboratory manuals Antisense DNA -- Laboratory manuals Molecular cloning -- Laboratory manuals Genetics -- Technique. Antisense DNA. Gene libraries. DNA probes. Nucleic acid probes. DNA. Nucleic acids. Molecular probes. Genetic Techniques DNA, Complementary Gene Library DNA, Single-Stranded DNA Probes Genetic Structures Investigative Techniques Nucleic Acid Probes DNA Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Phenomena Nucleic Acids Phenomena and Processes Molecular Probes Laboratory Chemicals Nucleic Acids, Nucleotides, and Nucleosides Chemicals and Drugs Specialty Uses of Chemicals Chemical Actions and Uses Genomic Library Nucleic acids Nucleic acid probes Molecular probes Genetics -- Technique DNA probes Antisense DNA DNA Gene libraries Molecular cloning DNA. Kloneren. Genetische manipulatie. Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Cowell, Ian G. Austin, Caroline A. ISBN 9781592595556 1592595553 089603383X 9780896033832 1280836768 9781280836763 9786610836765 6610836760

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