Title DNA viruses : methods and protocols / edited by Paul M. Lieberman

Published Totowa, N.J. : Humana Press, ©2005

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Description 1 online resource (xiv, 498 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 292 Methods in molecular biology (Clifton, N.J.) ; v. 292 Contents Viral detection -- Quantitative detection of Epstein-Barr virus DNA in clinical specimens by rapid real-time PCR targeting a highly conserved region of EBNA-1 -- Profiling of Epstein-Barr virus latent RNA expression in clinical specimens by gene-specific multiprimed cDNA synthesis and PCR -- Quantitative detection of viral gene expression in populations of Epstein-Barr virus-infected cells in vivo -- Detection and quantification of the rare latently infected cell undergoing herpes simplex virus transcriptional activation in the nervous system in vivo -- Reporter cell lines for the detection of herpes simplex viruses -- Unraveling the architecture of viruses by high-resolution atomic force microscopy -- Studying the structure of large viruses with multiresolution imaging -- Herpes simplex virus-cell interactions studied by low-fading contrasted immunofluorescence -- Herpes simplex virus-cell interactions studied by immunogold cryosection electron microscopy -- FTIR microscopy detection of cells infected with viruses -- The JC virus-like particle overlay assay -- Analysis of fusion using a virus-free cell fusion assay -- Pseudovirions as specific tools for investigation of virus interactions with cells -- Simultaneous in situ detection of RNA, DNA, and protein using tyramide-coupled immunofluorescence -- Identification and characterization of herpesviral immediate-early genes -- Methods for measuring the replication and segregation of Epstein-Barr virus-based plasmids -- DNA affinity purification of Epstein-Barr virus OriP-binding proteins Pre-B-cell colony formation assay -- Luciferase real-time bioluminescence imaging for the study of viral pathogenesis -- Culturing primary and transformed neuronal cells for studying pseudorabies virus infection -- Human papillomavirus type 31 life cycle: methods for study using tissue culture models -- Molecular genetics of herpesviruses: a recombinant technology approach -- Molecular genetics of DNA viruses: recombinant virus technology -- Genetic analysis of cytomegalovirus by shuttle mutagenesis -- Construction of a gene inactivation library for Bovine herpesvirus 1 using infectious clone technology -- Selective silencing of viral gene E6 and E7 expression in HPV-positive human cervical carcinoma cells using small interfering RNAs -- Design of a herpes simplex virus type 2 long oligonucleotide-based microarray: global analysis of HSV-2 transcript abundance during productive infection -- Real-time quantitative PCR analysis of viral transcription -- Rapid screening of chemical inhibitors that block processive DNA synthesis of herpesviruses: potential application to high-throughput screening Summary A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease Analysis dna viruses Virology Virologie Bibliography Includes bibliographical references and index Notes English Print version record Subject DNA viruses -- Laboratory manuals DNA viruses. Genetics -- Technique. Methodology. Microbiology. Viruses. Biology. Life sciences. Physical sciences. Virology. DNA Viruses Genetic Techniques Methods Microbiology Vertebrate Viruses Investigative Techniques Viruses Analytical, Diagnostic and Therapeutic Techniques and Equipment Organisms Biology Biological Science Disciplines Natural Science Disciplines Disciplines and Occupations Virology -- methods Virology methodology. microbiology. biology. biological sciences. physical sciences. SCIENCE -- Life Sciences -- Biology. SCIENCE -- Life Sciences -- Microbiology. DNA viruses Genre/Form Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Lieberman, Paul M ISBN 9781592598489 159259848X 1280358203 9781280358203 9786610358205 6610358206

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