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Title Human cytomegaloviruses : methods and protocols / edited by Andrew D. Yurochko, William E. Miller
Published New York : Humana Press, 2014

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Description 1 online resource (xi, 368 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1064-3745 ; 1119
Methods in molecular biology (Clifton, N.J.) ; v. 1119. 1064-3745
Contents History of the molecular biology of cytomegaloviruses / Mark F. Stinski -- Overview of human cytomegalovirus pathogenesis / Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko -- Distinct properties of human cytomegalovirus strains and the appropriate choice of strains for particular studies / Giada Frascaroli and Christian Sinzger -- Use of diploid human fibroblasts as a model system to culture, grow, and study human cytomegalovirus infection / Elizabeth A. Fortunato -- Use of recombinant approaches to construct human cytomegalovirus mutants / Iryna Dekhtiarenko, Luka Čičin-Šain, and Martin Messerle -- Use of primary human cells (fibroblasts, monocytes, and others) to assess human cytomegalovirus function / Emma Poole, Matthew Reeves, and John H. Sinclair -- Hematopoietic long-term culture (hLTC) for human cytomegalovirus latency and reactivation / Mahadevaiah Umashankar and Felicia Goodrum -- Analysis of cytomegalovirus binding/entry-mediated events / Gary C.T. Chan and Andrew D. Yurochko -- Use of 5-Ethynyl-2' -deoxyuridine labelling and flow cytometry to study cell cycle-dependent regulation of human cytomegalovirus gene expression / Lüder Wiebusch and Christian Hagemeier -- Methods for studying the function of cytomegalovirus GPCRs / Christine M. O'Connor and William E. Miller -- Methods for the detection of cytomegalovirus in glioblastoma cells and tissues / Charles S. Cobbs, Lisa Matlaf, and Lualhati E. Harkins -- Methods to study the nucleocytoplasmic transport of macromolecules with respect to their impact on the regulation of human cytomegalovirus gene expression / Marco Thomas, Barbara Zielki, Nina Reuter, Thomas Stamminger -- Fluorescence-based laser capture microscopy technology facilitates identification of critical in vivo cytomegalovirus transcriptional programs / Craig N. Kreklywich, Patricia P. Smith, Carmen Baca Jones, Anda Cornea, Susan L. Orloff and Daniel N. Streblow. -- Techniques for characterizing cytomegalovirus-encoded miRNAs / Lauren M. Hook, Igor Landais, Meaghan H. Hancock, and Jay A. Nelsonet -- What we have learned from animal models of HCMV / Pranay Dogra and Tim E. Sparer -- Rodent models of congenital cytomegalovirus infection / Djurdjica Cekinovic, Vanda Juranic Lisnic, and Stipan Jonjic -- Recent approaches and strategies in the generation of antihuman cytomegalovirus vaccines / Suresh B. Boppana and William J. Britt -- Approaches for the generation of new anti-cytomegalovirus agents : identification of protein-protein interaction inhibitors and compounds against the HCMV IE2 protein / Beatrice Mercorelli, Giorgio Gribaudo, Giorgio Palù and Arianna Loregian
Summary With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (SpringerLink, viewed May 28, 2014)
Subject Cytomegalovirus infections -- Laboratory manuals
Herpesvirus diseases.
Virus diseases.
DNA viruses.
Viruses.
Diseases.
Betaherpesvirinae
Herpesviridae Infections
Herpesviridae
DNA Virus Infections
Virus Diseases
DNA Viruses
Viruses
Disease
Vertebrate Viruses
Organisms
Cytomegalovirus
Cytomegalovirus Infections
illness.
disease.
Viruses
Virus diseases
Herpesvirus diseases
DNA viruses
Diseases
Cytomegalovirus infections
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Yurochko, Andrew D., editor.
Miller, William E., Professor, editor.
ISBN 9781627037884
1627037888
162703787X
9781627037877