Title Immunological tolerance : methods and protocols / edited by Paul J. Fairchild

Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (xiv, 477 pages) : illustrations (some color) Series Methods in molecular biology ; v. 380 Methods in molecular biology (Clifton, N.J.) ; v. 380 Contents Frontiers of immunological tolerance / Giogrgio Raimondi, Hēth R. Turnquist, and Angus W. Thomson -- Part I, Cell types contributing to immunological tolerance -- Balancing tolerance and immunity: the role of dendritic cell and t cell subsets / Elena Shklovskaya and Barbara Fazekas de St. Groth -- Differentiation of dendritic cell subsets from mouse bone marrow / Ludovica Bruno -- Genetic modification of dendritic cells through the directed differentiation of embryonic stem cells / Paul J. Fairchild, Kathleen F. Nolan, and Herman Waldmann -- Generation of immunocompetent t cells from embryonic stem cells / Renée F. de Pooter and Juan Carlos Zúñiga-Pflücker -- Isolation, expansion, and characterization of human natural and adaptive regulatory t cells / Silvia Gregori [and others] -- Derivation, culture, and characterization of thymic epithelial cell lines / Michiyuki Kasai and Toshiaki Mizuochi -- Part II, The study of immunological tolerance in vitro -- Thymus organogenesis and development of the thymic stroma / Craig S. Nowell, Alison M. Farley, and C. Clare Blackburn -- Generation of a tissue-engineered thymic organoid / Fabrizio Vianello and Mark C. Poznansky -- Studying t-cell repertoire selection using fetal thymus organ cultures / Philip G. Ashton-Rickardt -- Investigating central tolerance with reaggregate thymus organ cultures / Graham Anderson and Eric J. Jenkinson -- Estimating thymic function through quantification of t-cell receptor excision circles / Marie-Lise Dion, Rafick-Pierre Sékaly, and Rémi Cheynier -- Gene expression profiling of dendritic cells by microarray / Maria Foti, Paola Ricciardi-Carastagnoli, and Francesca Granucci -- SAGE analysis of cell types involved in tolerance induction / Kathleen F. Nolan, Stephen P. Cobbold, and Herman Waldmann -- Analyzing the physicodynamics of immune cells in a three-dimensional collagen matrix / Peter Reichardt, Frank Gunzer, and Matthias Gunzer -- Part III, The study of immunological tolerance in vivo -- Etiology of autoimmune disease: how t cells escape self-tolerance / Eli Sercarz and Claudia Raja-Gabaglia -- Animal models of spontaneous autoimmune disease: type 1 diabetes in the nonobese diabetic mouse / Nadia Giarratana, Giuseppe Penna, and Luciano Adorini -- Antigen-based therapy and immune regulation in experimental autoimmune encephalomyelitis / Mandy J. McGeachy [and others] -- Induction and regulation of inflammatory bowel disease in immunodeficient mice by distinct CD4+ t-cell subsets / Kevin J. Maloy -- In vivo models for the study of transplantation tolerance / Hanna Stewart, Rommel Ravanan, and Richard Smith -- Ectopic transplantation of tissues under the kidney capsule / Nathan J. Robertson, Paul J. Fairchild, and Herman Waldmann -- Intravital two-photon imaging of t-cell priming and tolerance in the lymph node / Susanna Celli and Philippe Bousso -- Tracing tolerance and immunity in vivo by CFSE-labeling of administered cells / Elizabeth Ingulli -- Part IV, Methods for inducing and breaking immunological tolerance -- Thymic involution: implications for self-tolerance / Frances T. Hakim and Ronald E. Gress -- Inducing mixed chimerism and transplantation tolerance through allogeneic bone marrow transplantation with costimulation blockade / Ines Pree and Thomas Wekerle -- Induction of dominant tolerance using monoclonal antibodies / Ana Água-Doce and Luis Graça -- Induction of tolerance by adoptive transfer of treg cells / Kanji Nagahama, Eiji Nishimura, and Shimon Sakaguchi -- Modulation of the immune response using dendritic cell-derived exosomes / Nicole R. Bianco [and others] -- Breaking self-tolerance to tumor-associated antigens by in vivo manipulation of dendritic cells / Ines Mende and Edgar G. Engleman Summary Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Immunological tolerance -- Laboratory manuals Dendritic cells -- Immunology -- Laboratory manuals Immunosuppression -- Laboratory manuals Immunological tolerance. Dendritic Cells -- immunology Laboratory Techniques and Procedures Immune Tolerance Models, Animal T-Lymphocytes -- immunology MEDICAL -- Immunology. Immunosuppression. Immune Tolerance. Dendritic Cells -- immunology. Laboratory Techniques and Procedures. Models, Animal. T-Lymphocytes -- immunology. Immunological tolerance. Immunological tolerance Immunosuppression Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Fairchild, Paul J. ISBN 1588296520 9781588296528 1597453951 9781597453950 1280971851 9781280971853 9786610971855 6610971854

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