| Description |
1 online resource (xvii, 446 pages) : illustrations (some color) |
| Series |
Springer protocols |
|
Methods in molecular biology, 1940-6029 ; 595 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 595.
|
| Contents |
Section I. Reviews.-- Review of human DC subtypes / Xnsheng J. Georgina Clark, Derek N.J. Hart.-- Review of murine dendritic cells: types, location, an development / Tewfik Miloud, Gunter J. Hammerling, Natalio Garbi.-- Section II. Human DC protocols.-- Isolation of human blood DC subtypes / Andrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, Kristen J. Radford.-- Isolation of immature primary langerhans cells from human epidermal skin / Marien A.W.P. de Jong, Lot de Witte, Teunis B.H. Geijtenbeek.-- Isolation and in vitro generation of gene-manipulated human plasmacytoid and conventional dendritic cells / Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen Spits, Bianca Blom.--̂In vivo modulation of gene expression by lentiviral transducation in "Human Immune System" Rag2-/-yc-/-mice / Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan M. Pouw, Kees Weijer, Hergen Spits, Bianca Blom, Nicolas Legrand.-- Generation of stable Th1/CTL-, Th2-, and Th17-inducing human dendritic cells / Pawel Kalinski, Eva Wiechowski, Ravikumar Muthuswamy, Esther de Jong.-- Pharmacological modification of dendritic cells to promote their tolerogenicity in transplantation / Heth R. Turnquist, Ryan T. Fischer, Angus W. Thomson.-- Towards a standardized protocol for the generation of monocyte-dendritic cell vaccines / Michael Erdmann, Beatrice Schuler-Thurner.-- Section III. Murine dendritic cell methods.-- CD8+, CD8-, and plasmacytoid dendritic cell generation in vitro using flt3 ligand / Shalin H. Naik, Meredith O'Keeffe, Anna Proietto, Hutertus Hochrein, Ken Shortman, Li Wu.--̂Generation of large numbers of pro-DCs and pre-DCs in vitro / Shalin H. Naik.-- Generation of plasmoacytoid and conventional dendritic cells with M-CSF / Meredith O'Keeffe, Ben Fancke, Hubertus Hochrein.-- Isolation of common dendritic cell progenitors (CDP) from mouse bone marrow / Nobuyuki Onai, Markus G. Manz, Michael A. Schmid.-- Isolation of mouse dendritic cells from lymphoid tissues and the indentification of dendritic cell subtypes by multiparameter flow cytometry / David Vremec.-- Isolation of cutaneous dendritic cells / Julie Helft, Miriam Merad.-- Isolation of skin dendritic cells from mouse and man / Patrizia Stoitzner, Nicolaus Romani, Alexander D. McLellan, Christoph H. Tripp, Susanne Ebner.-- Identification and isolation of rodent respiratory tract dentdritic cells / Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, Patrick G. Holt.-- Isolation of mouse thymic dendritic cell precursors / Li Wu, Angela D'Amico.--̂Isolation of rat intestinal lymph DC / Simon Milling, Gordon MacPherson.-- Mouse modesl of viral infection: influenza infection in the lung / Adele M. Mount, Gabrielle T. Belz.-- DCs in mouse models of intracellular bacterial infection / Michael Neuenhahn, Matthias Schiemann, Dirk H. Busch.-- Studying the function of dendritic cells in mouse models of asthma / Philippe Pouliot, Monique A. Willart, Hamida Hammad, Bart N. Lambrecht.-- Direct ex vivo activation of T cells for analysis of dendritic cells antigen presentation / Gabrielle T. Belz.-- Methods to study pulmonary dendritic cell migration / Claudia Jakubzick, Gwendalyn J. Randolph.-- Measuring pH, ROS production, maturation, and degradation in dendritic cell phagosomes using cytofluorometry-based assays / Ariel Savina, Pablo Vargas, Pierre Guermonprez, Anna-Maria Lennon, Sabastian Amigorena.-- Adaptive treg generation by DCs and their functional analysis / Li Wang.--̂Generation of parabiotic mice for the study of DC and DC precursor circulation / Claudia Waskov.-- Defining in vivo dendritic cell functions using CD11c-DTR transgenic mice / Liat Bar-On, Steffen Jung |
| Summary |
Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field |
| Analysis |
bloedcellen |
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blood cells |
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laboratoriummethoden |
|
laboratory methods |
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protocollen |
|
protocols |
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Cellular Biology |
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Celbiologie |
| Notes |
Title from PDF title page (SpringerLink, viewed December 7, 2009) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Dendritic cells -- Laboratory manuals
|
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Dendritic Cells -- cytology
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Dendritic Cells -- physiology
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Dendritic cells
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Naik, Shalin H
|
| ISBN |
9781607614210 |
|
1607614219 |
|
