| Description |
1 online resource (xi, 215 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 968 |
|
Methods in molecular biology (Clifton, N.J.) ; 968. 1064-3745
|
| Contents |
Overview of invasive fungal infections / Nina L. Tuite and Katrina Lacey -- Fungal diagnostics : review of commercially available methods / Javier Yugueros Marcos and David H. Pincus -- Rapid methods for the extraction and archiving of molecular grade fungal genomic DNA / Andrew M. Borman, Michael Palmer, and Elizabeth M. Johnson -- APEX DNA microarray for the identification of pathogenic fungi / Arianna Tavanti, Stefano Landi, and Sonia Senesi -- Microscopic detection of yeasts using fluorescence in situ hybridization / João Inácio and Maria da Luz Martins -- Quantitative detection of Aspergillus spp. by real-time nucleic acid sequence-based amplification / Yanan Zhao and David S. Perlin -- Differentiation of fungi using hybridization probes on the LightCycler® / Stephan Fricke [and others] -- Quantitative and multiplex detection of pathogenic fungi using padlock probes, generic qPCR, and suspension array readout / Magnus Jobs, Ronnie Eriksson, and Jonas Blomberg -- Species-specific identification of a wide range of clinically relevant fungal pathogens by the Luminex® xMAP technology / S. Preuner and T. Lion -- Applied gene histopathology : identification of Fusarium species in FFPE tissue sections by in situ hybridization / Yoichiro Okubo [and others] -- Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains / Orsolya Bouquet [and others] -- Surface plasmon resonance genosensor for the detection of Fusarium culmorum / Michelangelo Pascale, Francesco Zezza, and Giancarlo Perrone -- Hyperbranching rolling circle amplification, an improved protocol for discriminating between closely related fungal species / Jiufeng Sun and Sybren de Hoog -- Loop-mediated isothermal amplification-based detection of Fusarium graminearum / Ludwig Niessen -- Multiplex-tandem PCR for fungal diagnostics / Anna Lau, Keith Stanley, and Tania Sorrell -- Specific detection of Pneumocystis jirovecii in clinical samples by flow cytometry / Joana Barbosa [and others] |
| Summary |
Invasive fungal infections are a significant cause of morbidity and mortality. Over the past decade there has been a concerted effort to develop reliable methods for the detection of such infections. In Fungal Diagnostics: Methods and Protocols, expert researchers in the field detail method performance, ease of use and turnaround time of innovative diagnostic methods. Focusing mainly on fungal detection in clinical settings, the methods described could also be applied to alternative fields such as environmental testing, agriculture, food production and veterinary diagnostics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fungal Diagnostics: Methods and Protocols seeks to aid scientists into the further study of fungal detection |
| Analysis |
Life sciences |
|
Microbiology |
|
schimmels |
|
fungi |
|
medische mycologie |
|
medical mycology |
|
veterinaire mycologie |
|
veterinary mycology |
|
diagnostiek |
|
diagnostics |
|
moleculaire detectie |
|
molecular detection |
|
laboratoriummethoden |
|
laboratory methods |
|
protocollen |
|
protocols |
|
Microbiology (General) |
|
Agricultural Mycology (General) |
|
Microbiologie (algemeen) |
|
Landbouwkundige mycologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Diagnostic mycology -- Laboratory manuals
|
|
Mycology.
|
|
Diagnostic Techniques and Procedures
|
|
Mycology
|
|
Mycology
|
|
Diagnostic mycology
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
O'Connor, Louise, Dr.
|
|
Glynn, Barry.
|
| ISBN |
9781627032575 |
|
1627032576 |
|
1627032568 |
|
9781627032568 |
|
