Title Chromatin immunoprecipitation assays : methods and protocols / edited by Philippe Collas

Published New York, NY : Humana Press, ©2009

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Description 1 online resource (x, 267 pages) : illustrations Series Springer protocols Methods in molecular biology, 1064-3745 ; v. 567 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 567. 1064-3745 Contents The state-of-the-art of chromatin immunoprecipitation / P. Collas -- Characterization and quality control of antibodies used in ChIP assays / G. Goens [and others] -- The fast chromatin immunoprecipitation method / J. Nelson, O. Denisenko and K. Bomsztyk -- [u]ChIP : chromatin immunoprecipitation for small cell numbers / J.A. Dahl and P. Collas -- Fish 'n ChIPs : Chromatin immunoprecipitation in the zebrafish embryo / L.C. Lindeman [and others] -- Epitope tagging of endogenous proteins for genome-wide chromatin immunoprecipitation analysis / Z. Wang -- Flow cytometric and laser scanning microscopic approaches in epigenetics research / L. Szekvolgyi [and others] -- Serial analysis of binding elements for transcription factors / J. Chen -- Modeling and analysis of ChIP-ChIP experiments / R. Gottardo -- Use of SNP-arrays for ChIP assays : Computational aspects / E.M. Muro [and others] -- Damid : A methylation-based chromatin profiling approach / M. Abed [and others] -- Chromosome conformation capture (from 3C to 5C) and its ChIP-based modification / A. Gavrilov [and others] -- Determining spatial chromatin organization of large genomic regions using 5C technology / N.L. Berkum and J. Dekker -- Analysis of nascent RNA transcripts by chromatin RNA immunoprecipitation / A. Obrdlik and P. Percipalle -- Methyl DNA immunoprecipitation / J.-J. Goval and J. Magdalena -- Immunoprecipitation of methylated DNA / A.L. Sørensen and P. Collas Summary Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell's biology Analysis chromatine chromatin immunoprecipitatietests immunoprecipitation tests antilichamen antibodies dna rna nucleic acids biologische technieken biological techniques protocollen protocols Nucleic Acids Immunology Immunologie Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Chromatin -- Laboratory manuals Genetics -- Technique. Chromatin Immunoprecipitation Genetic Techniques Immunoprecipitation Immunologic Techniques Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetics -- Technique Chromatin Immunoprécipitation de la chromatine. Chromatin DNS-Chip Chromatine. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Collas, Philippe, 1966- ISBN 9781603274142 1603274146

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